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Nucleic Acid Data:
1 pmole of 1,000 b RNA = 0.33 µg
1 pmole of 1,000 bp DNA = 0.66 µg (3.03 pmoles of ends)
1 pmole of pUC18/19 DNA (2,686 bp) = 1.77 µg
1 pmole of lambda DNA (48,502 bp) = 32.01 µg
Molecular Weight Conversions:
MW of a double-stranded DNA molecule = (# of base-pairs) x (650 daltons/base-pair)
MW of a single-stranded DNA molecule = (# of bases) x (330 daltons/base)
MW of a single-stranded RNA molecule = (# of bases) x (340 daltons/base)
Estimating Tm of an Oligonucleotide:
Tm = [4°C x # of Cs and Gs in the oligo] + [2°C x # of As
and Ts in the oligo]
Estimating Molar Amounts of Primer:
To estimate the weight of 1 picomole of oligodeoxynucleotide, multiply
the number of nucleotides by 0.33 ng. For example, one picomole of
a 24-mer is 7.92 ng (24 x 0.33 ng = 7.92 ng).
Estimating Molar Amounts of Template:
To estimate the weight of 100 fmoles of double-stranded DNA template,
multiply the number of kilobases by 66 ng. For example, 100 fmoles
of a 2.7 Kb plasmid is 178 ng (2.7 x 66 ng = 178 ng).
| Template Required (fmoles) |
1 Kb |
3 Kb |
5 Kb |
7 Kb |
9 Kb |
| 25 |
17 ng |
50 |
83 |
120 |
150 |
| 50 |
33 |
100 |
165 |
230 |
300 |
| 75 |
50 |
150 |
250 |
350 |
450 |
| 100 |
66 |
200 |
330 |
460 |
600 |
| 150 |
100 |
300 |
500 |
700 |
900 |
| 200 |
135 |
400 |
660 |
930 |
1200 |
| 250 |
165 |
500 |
830 |
1200 |
1550 |
| 300 |
200 |
600 |
1000 |
1400 |
1800 |
Protein Yield Information
| Wet Weight |
9.5 x 10-13 g |
0.95 g |
| Dry Weight |
2.8 x 10-13 g |
0.28 g |
| Total Protein |
1.55 x 10-13 g |
0.15 g |
| Volume |
1.15 µm3 = 1 femtoliter |
|
| Protein concentration in the cell: 135 mg/ml |
0.1% of total protein |
150 µg/liter |
2.0% of total protein |
3 mg/liter |
50.0% of total protein |
75 mg/liter |
Buffer and Gel Tables
| 3.5 |
1,000 - 2,000 |
460 |
100 |
| 5.0 |
80 - 500 |
260 |
65 |
| 8.0 |
60 - 400 |
160 |
45 |
| 12.0 |
40 - 200 |
70 |
20 |
| 15.0 |
25 - 150 |
60 |
15 |
| 20.0 |
6 - 100 |
45 |
12 |
aN,N'-methylenebisacrylamide is included
at 1/30th the concentration of acrylamide.
bThe numbers given are the approximate sizes (in nucleotide
pairs) of fragments of double-stranded DNA with which the dye comigrates. |
| 4 |
>250 |
155 |
30 |
6 |
60 - 250 |
110 |
25 |
8 |
40 - 120 |
75 |
20 |
10 |
20 - 60 |
55 |
10 |
| aThe numbers given are the approximate sizes
(in nucleotide pairs) of fragments of double-stranded DNA with which
the dye comigrates. |
0.6 |
1,000 - 20,000 |
0.7 |
800 - 10,000 |
0.9 |
500 - 7,000 |
1/2 |
400 - 6,000 |
1.5 |
200 - 3,000 |
2.0 |
100 - 2,000 |
8% |
40 - 200 kDa |
10% |
21 - 100 kDa |
12% |
10 - 70 kDa |
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