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TargetAmp™ aRNA Amplification Kit Selection Guide

TargetAmp Selection Guide | TargetAmp Process | TargetAmp Performance | TargetAmp FAQs | TargetAmp Poster

The TargetAmp Kits set new standards in small sample RNA amplification...
because the best microarray data comes from small samples.

	One-Round Amplification			Two-Round Amplification
	TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101	TargetAmp™ 1-Round aRNA Amplification Kit 103	TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105*	TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0	TargetAmp™ 2-Round aRNA Amplification Kit 2.0	TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0
Starting Total RNA per Reaction	25–500 ng	25–500 ng	25–500 ng	10–500 pg	10–500 pg	50-500 pg
Typical Yield of Amplified aRNA	3–60 µg	3–60 µg	3–60 µg	1.5–70 µg	1.5–70 µg	5–70 µg
Reverse Transcriptase(s) Used	SuperScript™ III (provided by the user)	SuperScript™ III (provided by the user)	SuperScript™ III (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)
Fold Amplification	>5000	>5000	>5000	>5,000,000	>5,000,000	>5,000,000
Time Required	6 hours	6 hours	6 hours	2 days	2 days	2 days
End Product	Aminoallyl-aRNA	Unlabeled-aRNA	Biotin-aRNA	Aminoallyl-aRNA	Unlabeled-aRNA	Biotin-aRNA
Target Label Options	Biotin-NHS, Cy™-NHS, or other aminoreactive compound (provided by the user)	Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)	Biotin (Biotin-UTP is included in this kit)	Biotin-NHS, Cy™-NHS, or other aminoreactive compound (provided by the user)	Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)	Biotin (Biotin-UTP is included in this kit)

*Use the TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®
to prepare biotin-aRNA for use with Ilumina^® expression arrays.