RNA Amplification and Gene Expression Analysis | Kits for Amplifying mRNA

TargetAmp™ aRNA Amplification Kits

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The TargetAmp Kits set new standards in small sample RNA amplification...
because the best microarray data comes from small samples.

TargetAmp Citations

Purify RNA from FFPE tissue

Purify total RNA from 1 - 10,000 cells

Sensitive qRT-PCR from a single cell

Biotin-X-X-NHS for labeling aminoallyl-aRNA or aminoallyl-cDNA

The TargetAmp aRNA Amplification Kits set new standards in small sample RNA amplification for microarray studies. The kits, based on the linear RNA amplification process developed in the laboratory of Dr. James Eberwine, offer:

High yields of aRNA (cRNA) from small samples.
- Produce microgram amounts of aRNA (cRNA) from 10 pg (1 cell) of total RNA using the TargetAmp 2-Round aRNA Amplification Kits.
- Produce microgram amounts of aRNA (cRNA) from 25 ng of total RNA using the TargetAmp 1-Round aRNA Amplification Kits.
Fast and easy reactions.
Reproducible RNA amplification and microarray results.
High correlation with Affymetrix standard labeling procedure.
High quality results using Affymetrix GeneChip®, Illumina BeadChip and other commercial and self-spotted microarray platforms.

Choose the TargetAmp Kit for your needs

For more details, check the TargetAmp protocols	1-Round Amplification			2-Round Amplification
For more details, check the TargetAmp protocols	TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101	TargetAmp™ 1-Round aRNA Amplification Kit 103	TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105	TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0	TargetAmp™ 2-Round aRNA Amplification Kit 2.0	TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0
Starting Total RNA per Reaction	25 ng – 500 ng	25 ng – 500 ng	25 ng – 500 ng	10 pg – 500 pg	10 pg – 500 pg	50 pg - 500 pg
Typical Yield of Amplified aRNA	3 µg – 60 µg	3 µg – 60 µg	3 µg – 60 µg	1.5 µg – 70 µg	1.5 µg – 70 µg	5 µg – 70 µg
Reverse Transcriptase(s) Used	SuperScript™ III (provided by the user)	SuperScript™ III (provided by the user)	SuperScript™ III (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)
Fold Amplification	>5000	>5000	>5000	>5,000,000	>5,000,000	>5,000,000
Time Required	6 hours	6 hours	6 hours	2 days	2 days	2 days
End Product	Aminoallyl-aRNA	Unlabeled-aRNA	Biotin-aRNA	Aminoallyl-aRNA	Unlabeled-aRNA	Biotin-aRNA
Target Label	Biotin-NHS, Cy™-NHS, or other-NHS (provided by the user)	Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)	Biotin-aRNA (Biotin-UTP is included in this kit)	Biotin-NHS, Cy™-NHS, or other-NHS (provided by the user)	Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)	Biotin-aRNA (Biotin-UTP is included in this kit)

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Catalog No.	Size

TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101
TAA1R4910	10 Reactions
TAA1R4924	24 Reactions
Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA. Greater than 5000-fold amplification. Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).

TargetAmp™ 1-Round aRNA Amplification Kit 103
TAU1R5110	10 Reactions
TAU1R5124	24 Reactions
Produces microgram amounts of unlabeled-aRNA from 25 ng – 500 ng total RNA. Greater than 5000-fold amplification. Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).

TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105
TAB1R80510	10 Reactions
TAB1R80524	24 Reactions
Produces microgram amounts of biotin-aRNA from 25 ng – 500 ng total RNA. Greater than 5000-fold amplification. Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user). New low price!

TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0
TAA2R4910	10 Reactions
TAA2R4924	24 Reactions
Produces microgram amounts of Aminoallyl-aRNA from 10 pg – 500 pg total RNA. Greater than 5,000,000-fold amplification. Optimized for use with SuperScript™ III & SuperScript™ II Reverse Transcriptase (provided by the user).

TargetAmp™ 2-Round aRNA Amplification Kit 2.0
TAU2R5110	10 Reactions
TAU2R51224	24 Reactions
Produces microgram amounts of unlabeled-aRNA from 10 pg – 500 pg total RNA. Greater than 5,000,000-fold amplification. Optimized for use with SuperScript™ III & SuperScript™ II Reverse Transcriptase (provided by the user).

TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0
TAB2R71010	10 Reactions
TAB2R71024	24 Reactions
Produces microgram amounts of biotin-aRNA from 50 pg – 500 pg total RNA. Greater than 5,000,000-fold amplification. Optimized for use with SuperScript™ III & SuperScript™ II Reverse Transcriptase (provided by the user).