EPICENTRE Biotechnologies
EPICENTRE Biotechnologies  
  Log In

TargetAmp™ aRNA Amplification Kit Selection Guide

TargetAmp Selection Guide | TargetAmp Process | TargetAmp Performance | TargetAmp FAQs | TargetAmp Poster | TargetAmp Citations

The TargetAmp Kits set new standards in small sample RNA amplification...
   because the best microarray data comes from small samples.

  One-Round Amplification Two-Round Amplification
TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101 TargetAmp™ 1-Round aRNA Amplification Kit 103 TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 105* TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 TargetAmp™ 2-Round aRNA Amplification Kit 2.0 TargetAmp™ 2-Round Biotin-aRNA Amplification Kit 3.0
Starting Total RNA per Reaction 25–500 ng 25–500 ng 25–500 ng 10–500 pg 10–500 pg 50-500 pg
Typical Yield of Amplified aRNA 3–60 µg 3–60 µg 3–60 µg 1.5–70 µg 1.5–70 µg 5–70 µg
Reverse Transcriptase(s) Used SuperScript™ III (provided by the user) SuperScript™ III (provided by the user) SuperScript™ III (provided by the user) SuperScript™ III & SuperScript™ II (provided by the user) SuperScript™ III & SuperScript™ II (provided by the user) SuperScript™ III & SuperScript™ II (provided by the user)
Fold Amplification >5000 >5000 >5000 >5,000,000 >5,000,000 >5,000,000
Time Required 6 hours 6 hours 6 hours 2 days 2 days 2 days
End Product Aminoallyl-aRNA Unlabeled-aRNA Biotin-aRNA Aminoallyl-aRNA Unlabeled-aRNA Biotin-aRNA
Target Label Options Biotin-NHS, Cy™-NHS, or other aminoreactive compound (provided by the user) Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user) Biotin (Biotin-UTP is included in this kit) Biotin-NHS, Cy™-NHS, or other aminoreactive compound (provided by the user) Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user) Biotin (Biotin-UTP is included in this kit)

*Use the TargetAmp™ Nano-g™ Kit to prepare biotin-aRNA for use with Ilumina® expression arrays.


HOME | PRODUCTS | WHAT'S NEW | ORDERING | TECHNICAL RESOURCES | CONTACT US | ABOUT US

Bookmark and Share

EPICENTRE® Biotechnologies - Understanding the Genome and Transcriptome
©2010 EPICENTRE Biotechnologies. All Rights Reserved.