The RiboZero™ family of kits remove >99% of the
ribosomal RNA (rRNA) from intact and degraded total RNA samples. Kits
are now available in a convenient magnetic format.
Note: The nonmagnetic versions of the Ribo-Zero kits
will be discontinued effective June 30, 2012.
If you've used a Ribo-Zero kit for a species not listed
here, e-mail your results to techhelp@epicentre.com,
and we'll send you a free Epicentre 2 GB flash drive.
| Human |
>99% removal of 28S, 18S, 5.8S and 5S rRNAs |
| Mouse |
>99% removal of 28S, 18S, 5.8S and 5S rRNAs |
| Rat |
>99% removal of 28S, 18S, 5.8S and 5S rRNAs |
| Other mammalian (e.g., cow, pig) |
Not tested but expected to perform well. |
| Drosophila |
Will not remove 5S rRNA or 'right' fragment of 28S rRNA. |
| C. elegans |
Use highest quality RNA possible. |
| Yeast |
Removes 26S, 18S, 5.8S rRNAs from intact sample. Will not remove
5S rRNA. Use highest quality RNA possible. |
| Xenopus |
Use highest quality RNA possible. |
| Mosquito |
Not recommended. Poor removal of 28S and 5S rRNAs. |
| Zebrafish |
Efficient removal of 28S, 18S, and 5S rRNAs. 5.8S rRNA removal
has not been tested. |
| Lamprey |
Use highest quality RNA possible. |
| Shrimp |
Not recommended |
| Aspergillus niger |
300 nt region of 18S rRNA will not be removed from a degraded sample.
Use highest quality RNA possible. |
| Nematostella vectensis |
Use highest quality RNA possible. |
| Dog |
Efficient removal of all rRNAs. |
| Plasmodium falciparum |
Removes 28S and 18S rRNA. Will not remove 5.8S or 5S rRNAs. |
| Mustela putorius (ferret) |
Efficient removal of rRNAs |
| Cynomolgus macaques (Macaque monkey) |
Efficient removal of rRNAs |
| Chlorocebus sabaeus (African green monkey) |
Efficient removal of rRNAs |
| Apis mellifera (Honey bee) |
Use highest quality RNA possible. |
| Notophthalmus viridescens(newt) |
Expected to remove 18S and 28S rRNA. May not remove 5.8S or 5S
rRNAs. Use highest quality RNA possible. |
| Schizosaccharomyces pombe |
Expected to remove all rRNA species from intact RNA sample. |
| E. coli |
>99.9% removal of 23S, 16S and 5S rRNAs |
| Lactobacillus plantarum |
>99% removal of 23S, 16S and 5S rRNAs |
| Salmonella typhimurium |
Efficiently removes 16S and 5S rRNAs. Does not remove a 107 nt
region of the fragmented 23S rRNA. |
| Campylobacter jejuni |
Efficiently removes 23S and 16S rRNAs from intact RNA. Will not
remove 5S rRNA. |
| Pseudomonas aeruginosa, putida |
Efficiently removes 23S, 16S and 5S rRNAs. |
| Porphyromonas gingivalis |
Efficiently removes 23S, 16S rRNAs. Will not remove 5S rRNA. |
| Cyanobacteria |
Efficiently removes 23S, 16S and 5S rRNAs. |
| Photorhabdus |
Efficiently removes 23S, 16S and 5S rRNAs. |
| Francisella |
Expected to efficiently remove 23S, 16S, 5S rRNAs. |
| Burkholderia pseudmoallei |
Expected to efficiently remove 23S, 16S, 5S rRNAs. |
| Azotobacter vinelandii |
Expected to efficiently remove 23S, 16S, 5S rRNAs. |
| Chromohalobacter |
Expected to efficiently remove 23S, 16S, 5S rRNAs. |
| Acinetobacter baumanii |
Efficiently remove 23S, 16S, 5S rRNAs. |
| Bacillus subtilis |
Removes >99% of 23S, 16S and 5S rRNAs. |
| Mycobacterium tuberculosis |
Efficiently removes 23S, 16S and 5S rRNAs. |
| Streptococcus |
Expected to efficiently remove 23S, 16S, 5S rRNAs. |
| Clostridium difficile |
Expected to efficiently remove 23S, 16S, 5S rRNAs. |
| Mycoplasma gallisepticum |
Removes 23S and 16S rRNAs. Will not remove 5S rRNA. |
| Methanoseata concilii |
Removes 23S and 16S rRNAs from intact RNA. Will not remove 5S rRNA.
Use highest quality RNA possible. |
| Synechococcus |
Removes 23S and 16S rRNAs from intact RNA. Will not remove 5S rRNA.
Use highest quality RNA possible. |
| Staphylococcus aureus |
Removes 23S, 16S and 5S rRNAs. |
| Methanococcus maripaludis |
Removes 23S and 16S rRNAs from intact RNA. Will not remove 5S rRNA.
Use highest quality RNA possible. |
| Deinococcus radiodurans |
Use highest quality RNA possible. |
| Geobacter metallireducens |
Expected to work well. May not remove 5S rRNA. Use highest quality
RNA possible. |
Sulfolobus acidocaldaris
Sulfolobus islandicus |
Expected to work well. Use highest quality RNA possible. |
| Mesotoga |
Expected to work well. Use highest quality RNA possible. |
| Methanobacterium |
Expected to work well. May not remove 5S rRNA. Use highest quality
RNA possible. |
