The EZ-Tn5™ Custom Transposome™ Construction Kits contains
all the reagents needed to build and use an EZ-Tn5 transposon in either
an in vivo or in vitro insertion reaction. Using standard
cloning methods and the included pMOD-2 or pMOD-3 Transposon vectors,
you can create custom transposons with almost any DNA. Prepare a transposon
complex with the included EZ-Tn5 Tranposase for in vivo experiments,
or mix the transposon with a suitable target DNA (plasmid or purified
genomic DNA) for an in vitro insertion reaction. The EZ-Tn5 Custom
Transposome Construction Kit is provided with sufficient reagents for
20 in vivo Transposome or 10 in vitro insertion reactions.
Figure 1 (click to enlarge). Overview of the procedure for
the EZ-Tn5™ Custom Transposome™ Construction Kits.