Applications

• In vivo mutagenesis or strain development of microorganisms.
• Plasmid/gene rescue.
• DNA sequencing.
• Gene expression studies.

The EZ-Tn5™ Custom Transposome™ Construction Kits contains all the reagents needed to build and use an EZ-Tn5 transposon in either an in vivo or in vitro insertion reaction. Using standard cloning methods and the included pMOD-2 or pMOD-3 Transposon vectors, you can create custom transposons with almost any DNA. Prepare a transposon complex with the included EZ-Tn5 Tranposase for in vivo experiments, or mix the transposon with a suitable target DNA (plasmid or purified genomic DNA) for an in vitro insertion reaction. The EZ-Tn5 Custom Transposome Construction Kit is provided with sufficient reagents for 20 in vivo Transposome or 10 in vitro insertion reactions.

Benefits

• Choice of two transposon construction vectors.
• Broad host range with over 60 Gram-negative or Gram-positive species documented.
• EZ-Tn5 Transposase and reaction buffers included for in vivo or in vitro transposition reactions.