Improved Technologies for Ribosomal RNA Removal and Directional RNA-Seq Library Preparation (1.1M PDF poster)

The Ribo-Zero™ Magnetic Kit (Plant Leaf) and Ribo-Zero™ Magnetic Kit (Plant Seed/Root) remove virtually all the ribosomal RNA (rRNA) from intact and partially degraded plant leaf, seed, and root total RNA samples for RNA-Seq sample prep and other applications.

Benefits

• Removes cytoplasmic, chloroplast and mitochondrial rRNA from intact and partially degraded plant leaf, seed, and root total RNA preparations.
• Single-pass process.
• Recovers mRNAs and large non-coding non-rRNAs.
• Recovers both the 5´- and 3´-end fragments of RNAs in a partially degraded RNA sample.
• Effective rRNA removal from a broad range of plant species.
• Automation-friendly magnetic bead process.

Table 1. Removal of plant rRNA using the Ribo-Zero™ Magnetic Kit (Plant Leaf) and Ribo-Zero™ Magnetic Kit (Plant Seed/Root). Five micrograms of the indicated RNA were treated using the appropriate Ribo-Zero Kit. The amount of residual rRNA in the Ribo-Zero-treated RNA was determined by random-primed qRT-PCR using PCR primers to the small rRNAs and to multiple regions of the large rRNAs. Percent rRNA removal was calculated from rRNA-specific ΔCt values, normalized to those of the EF-1α transcript.

Figure 2. Agilent Bioanalyzer analysis of RNA treated with the Ribo-Zero™ Magnetic Kit (Plant Seed/Root). Bioanalyzer (RNA600 Pico Chip) profiles of 1 µg and 5 µg of soy root RNA before (green) and after (blue and red) a Ribo-Zero™ (Plant Seed/Root) reaction.