The Globin-Zero™ Gold Kit enriches for non-globin coding and non-coding RNAs in blood-derived RNA samples. A Globin-Zero kit reaction efficiently removes the highly abundant adult globin mRNAs and the cytoplasmic and mitochondrial rRNAs from RNA isolated from human, mouse or rat blood for RNA-Seq library preparation and other applications.

Benefits

• Sequence deeper & discover more: Enrich for coding and non-coding RNAs for deeper reads into your blood RNA samples.
• More informative RNA-Seq. Retain non-globin mRNAs and large non-ribosomal, non-coding RNAs for more informative whole transcriptome RNA-Seq.
• Compromised sample…no problem. Efficient globin mRNA and rRNA removal from both intact and partially degraded samples.
• Save time and sample. Rapid 90-minute, single-pass process saves time and sample compared to multi-step, multi-kit procedures.
• Automation-friendly. Magnetic bead format enables manual or automated processing.

Table 1. A Globin-Zero™ Gold Kit reaction removes both globin mRNAs and ribosomal RNAs from blood RNA samples.

Figure 1. Enrich for coding and non-coding RNAs. Five micrograms of DNA-free RNA isolated from human whole blood (A) and erythroid cells (B) were treated with the Globin-Zero Gold Kit. RNA-Seq libraries were made from the Globin-Zero treated RNA using Epicentre's ScriptSeq™ v2 RNA-Seq Library Preparation Kit and sequenced on an Illumina® GAII. Fewer than 2% of the sequencing reads in each library correspond to globin mRNA + rRNA.