The ScriptSeq™ Complete Kit (Bacteria) combines Epicentre's powerful Ribo-Zero™ technology and rapid ScriptSeq v2 RNA-Seq library prep procedure for optimized comprehensive preparation of RNA-Seq libraries in 1 day (Fig. 1). The ScriptSeq Complete Kit (Bacteria) requires 1-5 µg and the ScriptSeq™ Complete Kits (Bacteria) - Low Input utilizes 100 ng - 1 µg of total RNA.
The ScriptSeq Complete Kit (Bacteria) includes Ribo-Zero™ rRNA removal reagents for removal of 23S, 16S and 5S rRNA from Gram-positive and Gram-negative bacterial total RNA (Fig. 2), and ScriptSeq v2 RNA-Seq Kit for directional RNA-Seq.