Note: For RNA-Seq applications, we recommend using a Ribo-Zero™ Kit.
Eukaryotic mRNA that is 3´-polyadenylated has traditionally been isolated
by elution from immobilized oligo(dT) or oligo(dU) substrates. The mRNA-ONLY™ Eukaryotic
mRNA Isolation Kit* provides a simple and effective method for isolating eukaryotic
mRNA that is substantially free of ribosomal RNA (rRNA), in less than an hour,
without binding to an oligo(dT) substrate.
The mRNA-ONLY Kit includes Epicentre's Terminator™* 5´-Phosphate-Dependent
Exonuclease, a processive 5´→3´ exonuclease that digests RNA
having a 5´ monophosphate. The enzyme does not digest RNA having a 5´ triphosphate,
a 5´ cap (present on most eukaryotic mRNAs), or a 5´-hydroxyl group.
These enzymatic properties make Terminator Exonuclease ideal for producing
mRNA-enriched samples from eukaryotic total RNA preparations by selectively
digesting the rRNAs (which have a 5´ monophosphate). Thus, the mRNA-ONLY
Eukaryotic mRNA Isolation Kit can be used to isolate eukaryotic mRNA substantially
free of 5.8S, 18S, and 28S rRNA. Transfer RNA (tRNA), and 5S rRNA are not removed
by Terminator Exonuclease digestion.
*Covered by issued and/or pending patents.