MasterPure™ Yeast RNA Purification Kit

Poster from RNA Society (714k)

Applications

  • Purification of RNA from yeast and filamentous fungi suitable for cDNA synthesis and microarray gene expression analysis (Figs. 1 and 2).

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi. The kit uses a rapid salting-out process1 to remove contaminating macromolecules, avoiding toxic organic solvents, bead-beating, and spheroplasting.2

Benefits

  • Avoids hot acid phenol.
  • Faster than spheroplasting.
  • Higher quality RNA than bead-beating.
  • No extra enzymes or equipment to purchase.
  • Yields 25-50 µg RNA from 1 ml of mid-log S.cerevisiae.

References

  1. Miller, S.A. et al., (1988) Nucleic Acids Res. 16, 1215.
  2. Hoffman, L.M. and Jarvis, B.W. (2004) Epicentre Forum 11(2), 4.
Figure 1. Microarray analysis of yeast RNA purified with the MasterPure™ Yeast RNA Purification Kit. The RNA was reverse transcribed, labeled, and hybridized to a S. cerevisiae DNA microarray. The cDNA from cells under oxidative stress was labeled with Cy5, and cDNA from control cells was labeled with Cy3. Figure 2. Purity of RNA obtained using the MasterPure™ Yeast RNA Purification Kit. The RNA used in Fig. 1. was stored at -20° C for two months before analysis on an Agilent Technologies 2100 Bioanalyzer®. The electrophoretogram demonstrates the high-purity, intact RNA.