Clear the lysate:
Vacuum filtration or
centrifugation, 1 min.
poster 2008, 2.2M PDF
The Direct Lysis Fosmid96 Kit isolates fosmid DNA by direct lysis of a 200-µl
overnight culture, eliminating the need for centrifugation of the culture and
resuspension of the cell pellet (Fig. 1). The kit is designed for rapid preparation
of sequencing templates in a 96-well format from fosmid clones (Figs. 2 and
3). The kit uses a modified alkaline lysis procedure that incorporates a proprietary
RNase Blend to remove the RNA efficiently, and an autoinduction solution for
higher cell growth and DNA yield when used with CopyControl™ fosmid clones.
The typical DNA yield from a 200-µl overnight autoinduced culture of a
CopyControl fosmid is sufficient for six to eight end-sequencing reactions.
The kit includes reagents and plastics for high-throughput DNA extraction,
and is suitable for robotic automation procedures.