Application note: Direct testing of human buccal cells
The QuickExtract™ DNA Extraction Solution can be used to rapidly and
efficiently extract PCR-ready genomic DNA from almost any sample type using
a simple, one-tube protocol that takes only 3-8 minutes (Fig. 1), depending
on the sample. QuickExtract Solution has been used to extract DNA from samples
such as hair follicles, quill-end cells of feathers, tissue-culture cells, buccal
cells, zebrafish organs and scales, and mouse tail snips. The extracted DNA
is suitable for PCR analysis (Fig. 2), such as genomic, transgenic, or viral
DNA screening in animals, or for genetic or environmental research and screening
in humans and other organisms.
The QuickExtract method allows for the inexpensive processing of one to hundreds
of samples simultaneously, without centrifugation, spin columns or the use of
any toxic organic solvent. The method is also compatible with robotic automation.