Ribonuclease H (RNase H) from E. coli is an endonuclease that specifically
degrades the RNA in an RNA:DNA hybrid, without affecting DNA or unhybridized
RNA. It will not degrade dsDNA, ssDNA, dsRNA, or ssRNA. Epicentre's RNase H
is highly purified and suitable for use in diagnostic probe research, as well
as other applications. However, in contrast to Hybridase™ Thermostable
RNase H, which can be used at temperatures up to 95°C, E. coli RNase
H is inactivated at 65°C in 10 minutes.
Unit Definition: One unit of RNase H results in the acid-solubilization
of 1 nmol of polyadenylic acid in the presence of an equimolar concentration
of polythymidylic acid in 20 minutes at 37°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1
M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Quality Control: RNase H is tested for the specific degradation of
RNA hybridized to DNA and is free of detectable exo- and endodeoxyribonuclease,
and non-RNase H RNase activities.