Epicentre's Uracil-DNA Excision Mix is a blend of enzymes that cleave DNA
at positions where uracil is present in place of thymine. The Uracil-DNA Excision
Mix is useful for specific or random cleavage of DNA or for DNA repair studies,
allowing mapping of uracil residues in any DNA. Uracil-DNA glycosylase in the
Excision Mix removes uracil bases from DNA, creating a single-base gap and
leaving the deoxyribose phosphate backbone intact. Endonuclease IV in the Excision
Mix then cleaves the DNA at each abasic site, leaving a 3´-hydroxyl end
and an abasic 5´-phosphorylated end. The minimum oligomer size that will
serve as a substrate is six base pairs. Uracil-DNA Excision Mix digestion products
can be analyzed by denaturing agarose gel electrophoresis or denaturing polyacrylamide
Uracil Excision Enzyme 10X Buffer: 500 mM Tris-HCl (pH 9.0), 200 mM
(NH4)2SO4, and 100 mM EDTA.
Quality Control: Uracil Excision Enzyme Mix activity is tested for
activity on a variety of DNA templates, and is free of detectable RNase and
nonspecific DNase activities.