Lambda Exonuclease is a highly processive 5´→3´ exodeoxyribonuclease
that selectively digests the 5´ phosphorylated strand of dsDNA.
The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA.
The enzyme has reduced activity against nicked DNA and against ssDNA
and gapped DNA.3
Unit Definition: One unit of Lambda Exonuclease will produce
10 nmol of acid-soluble deoxyribonucleotides from dsDNA template in 30
minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer.
Quality Control: Lambda Exonuclease is function-tested to ensure
complete and preferential degradation of PCR products produced using
5´-phosphorylated primers. PCR products made using primers containing
5´-OH ends are not digested. Lambda Exonuclease is tested to be
free of contaminating endonuclease activities.