RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing
DNA that might interfere with the characterization, manipulation, or
use of RNA, or for any application requiring highly purified DNase I,
such as nick translation. It efficiently hydrolyzes dsDNA and ssDNA into
a mixture of short oligonucleotides and mononucleotides.
Unit Definition: One Molecular Biology Unit (MBU) of RNase-Free
DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10
minutes at 37°C under standard assay conditions.
Storage Buffer: 50% glycerol containing 10 mM Tris-HCl (pH 7.5),
10 mM CaCl2, and 10 mM MgCl2.
DNase I 10X Reaction Buffer: 100 mM Tris-HCl (pH 7.5), 25 mM MgCl2,
and 5 mM CaCl2.
Quality Control: No degradation of 1 µg of a synthetic
RNA transcript is detected by agarose gel electrophoresis following incubation
with 10 U of RNase-Free DNase I at 37°C for 1 hour.