Applications

• DNA repair research.
• Antitumor drug evaluation.

Endonuclease IV, cloned from the E. coli nfo gene, is a metalloenzyme that functions in vivo to repair free-radical damage in DNA.¹ The enzyme has Class II abasic endonuclease activity, which has utility in many areas of DNA damage and repair research.¹ The enzyme can also act as an 3´ diesterase. Endonuclease IV is useful in the study of the effects of antitumor drugs such as bleomycin on nucleic acids in vivo.²

Unit Definition: One unit of Endonuclease IV will relax 1 µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 minutes at 37°C under standard assay conditions.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1 mM DTT, and 0.1% Triton® X-100.

Quality Control: Endonuclease IV is tested in degradation of dsDNA and is free of detectable RNase and double-stranded exonuclease activities.

References

1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711.
2. Levin, J.D. and Demple, B. (1996) Nucleic Acids Res. 24, 885.