The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated
to use high concentrations of NTPs, that can be inhibitory to other in vitro transcription
systems. AmpliScribe High Yield Transcription reactions can produce >20-fold
more full-length RNA transcript than conventional in vitro transcription
reactions. For example, the AmpliScribe T7 Kit yields approximately 5-7.5 mg/ml
of 1.4-kb full-length control RNA in 30-120 minutes, respectively, using 1 µg
of template DNA (Fig. 1).
Note: Because of the very high concentration of NTPs used in AmpliScribe
reactions, these kits are not recommended for preparing high specific-activity
radiolabeled RNA probes.