End-It™ DNA End-Repair Kit

Applications

  • Repair sheared, nebulized, or restriction enzyme-digested genomic DNA for: preparation of templates for next-gen sequencing; or shotgun library preparation.

The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit.

The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).

Benefits

  • The repaired DNA is blunt-ended and 5´-phosphorylated for immediate blunt-end ligation.
  • The high specific activity of the End-Repair Enzyme Mix provides complete conversion of protruding ends to 5´-phosphorylated, blunt-ended DNA.
Figure 1. DNA fragments containing any type of ends are rapidly and efficiently converted to 5´-phosphorylated, blunt-ended DNA using the End-It™ DNA End-Repair Kit. The end-repaired DNA is ready for blunt-end ligation using, for example Epicentre's Fast-Link™ DNA Ligation Kit.