MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging
efficiencies of cos site-containing methylated or unmethylated DNA. Traditional
lambda packaging extracts are derived from two complementary lysogenic E.
coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax
Extracts use a restriction-free E. coli K-12 strain, NM759, in place
of strain BHB2690.2 The sonicated extracts of NM759 are combined
with extracts of BHB2688 to produce MaxPlax Extracts.
Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging
efficiency of greater than 1 x 109 pfu/µg of Control Ligated
Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate
of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided
with each lot of MaxPlax Extracts.