Fast PCR can be achieved using fast PCR instrumentation or by using enzyme/reagent
combinations that improve PCR efficiency. With a standard thermocycler, the
TAQXpedite™ PCR system achieves fast PCR results with total reaction
time as little as 16 minutes for a 500-bp amplicon (Table 1, Fig. 1). The
TAQXpedite™ kit can be used with a either a specialized fast PCR thermocycler
or a standard thermocycler.
The TAQXpedite PCR System (FAST End-Point) contains a unique blend of thermostable
DNA polymerases that can be used for fast PCR, a carefully optimized 2X Universal
MasterMix and a Difficult/Long MasterMix with all four dNTPs, and an optimized
MgCl2 concentration. The MasterMix also contains Epicentre's patented
PCR Enhancer with betaine,* which substantially improves the yield, efficiency,
and specificity of amplification of many target sequences, especially those
containing a high GC content or secondary structure.1-5 The TAQXpedite
PCR System's Universal Master-Mix is designed for routine and/or high-throughput
fast PCR applications (Fig. 1), while the Difficult/Long MasterMix is designed
for use with troublesome, non-routine, or long applications (Fig. 2). All
reaction components are included in the kit except for template and primers.
Table 1. Total amplification times for TAQXpedite™ and standard
TAQXpedite™ PCR System. Purchase of this product includes an immunity
from suit under patents specified in the product insert to use only the amount
purchased for the purchaser's own internal research. No other patent rights
(such as 5´ Nuclease Process patent rights) are conveyed expressly, by
implication, or by estoppel. Further information on purchasing licenses may
be obtained by contacting the Director of Licensing, Applied Biosystems, 850
Lincoln Centre Drive, Foster City, California 94404, USA.
*Covered by issued and/or pending patents.