RepliPHI™ Phi29 DNA Polymerase (φ29 DNA Polymerase)
is a highly processive enzyme with exceptional strand-displacement activity.
The enzyme also contains a 3´→5´ exonuclease activity
that enables proofreading capability.
Specific Activity: 1 x 106 Units/mg.
Storage Buffer: RepliPHI™ Phi29 DNA Polymerase is supplied
in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM
NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5% NP-40, and 0.5% Tween 20.
Unit Definition: One unit of RepliPHI™ Phi29 DNA Polymerase
converts 25 pmol of dNTPs into acid-insoluble material in 30 minutes
at 30°C under standard assay conditions.
Reaction Buffer: A Reaction Buffer is not supplied with RepliPHI
Phi29 DNA Polymerase; however, a suggested Reaction Buffer is 40 mM Tris-HCl
(pH 7.5), 50 mM KCl, 10 mM MgCl2, 5 mM (NH4)2SO4,
and 4 mM DTT. A 10X Reaction Buffer is supplied with the RepliPHI Phi29
Quality Control: RepliPHI Phi29 DNA Polymerase is free of detectable
endonuclease and RNase activities. RepliPHI Phi29 DNA Polymerase is free
of detectable DNA contamination.