High Throughput and High Quality BAC DNA

Purify 384 fosmids or BACs in less than four hours with EPICENTRE’s BACMAX96™ DNA Purification Kit. The BACMAX96™ DNA Purification Kit was developed for the easy and reliable isolation of high-quality BAC and fosmid DNA in a 96-well format. The BACMAX96 Kit consistently yields up to 900 ng of DNA from single-copy BAC and fosmid clones, and from 2.5 µg to 6 µg of DNA from the CopyControl™ BAC and fosmid clones induced to high copy number. The unprecedented yields from the CopyControl clones are due in part to an autoinduction protocol that was specifically designed for a high-throughput format. Purified DNA performs excellently in downstream applications such as fingerprinting, end sequencing, and transposon insertion sequencing.