Clone Unstable DNA

EPICENTRE Biotechnologies’ CopyCutter™ EPI400™ E. coli cells significantly lower the copy number of many common vectors so that “toxic” genes or unstable DNA sequences are easier to clone. The copy number of vectors containing ColE1- and pMB1-type origins that are present in various derivatives of pUC, pBR, pET and many other vectors is reduced up to 25-fold, increasing the likelihood that an insert will be stabley maintained. The highly toxic regB gene, for example, was successfully cloned into a pET11 vector only when recombinants were maintained in CopyCutter cells.