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EPICENTRE's revolutionary Nextera™ technology*
uses in vitro transposition to prepare sequencer-ready libraries
from genomic DNA for multiple sequencing platforms. The technology simultaneously
fragments and tags DNA, in a single-tube reaction.
- Use nanogram amounts of starting DNA.
- Prepare sequencer-ready libraries in less than 2
hours.
- Incorporate platform-specific tags and optional barcodes.
- Validated on Roche 454™ GS FLX and GS FLX Titanium™,
and Illumina Solexa® GAI, GAII.
| Fragmentation |
✓ (15-30 min) |
Add Nextera™ Enzyme
Mix
(5 min) |
| Collection |
✓ (15 min) |
| Concentration |
✓ (15
min) |
| Size Selection |
✓ (60
min) |
| End-Repair |
✓ (60
min) |
| Clean-Up |
✓ (15
min) |
| A-Tailing |
+/- (30
min) |
| Adaptor Ligation |
✓ (60
min) |
| Clean-Up |
✓ (15
min) |
✓ (15
min) |
| Library Enrichment |
Variable (~60
min) |
PCR (~60
min) |
For more information:
Product Information and ordering:
*Covered by issued and/or pending patents.
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