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Epicentre Forum 6 (2)
Epicentre's EZ::TN™ Transposon Tools offer a variety of kits for
the many in vitro and in vivo applications. Have questions about what
kit is best for your needs? Please contact our technical
consultants for assistance.
Use these kits for inserting sequencing primer binding sites, or for
creating gene "knockouts" in plasmid, cosmid, and BAC clones in vitro.
EZ::TN™ <KAN-1> Insertion Kit EZI911K 10
Reactions
Inserts primer binding sites and the kanamycin resistance marker into
target DNA in vitro. The kit includes EZ::TN <KAN-1> Transposon,
EZ::TN Transposase, buffer, and unlabeled primers. Generates >106 kanamycin-resistant
transformants per reaction.
EZ::TN™ <TET-1> Insertion Kit EZI921T 10
Reactions
Inserts primer binding sites and the tetracycline resistance marker
into target DNA in vitro. The kit includes EZ::TN <TET-1> Transposon,
EZ::TN Transposase, buffer, and unlabeled primers. Generates >106 tetracycline-resistant
transformants per reaction.
Use this kit for inserting primer binding sites and selectable markers,
or for creating gene "knockouts" in bacterial DNA in vivo by direct
electroporation into living bacteria.
EZ::TN™<KAN-1>Tnp Transposome™ Kit TSM9301 10 in
vivo Reactions
Create gene "knockouts," insert primer binding sites and the kanamycin
resistance marker into bacterial chromosomal or extra-chromosomal DNA
by electroporation into living bacteria.
These kits are used for creating nested segment deletions and inversions
in plasmid or cosmid clones in vitro. Clone your DNA of interest
into one of the specially constructed plasmid or cosmid cloning vectors
supplied with the respective kit. Create sequence deletions and inversions in
vitro via intramolecular transposition by adding transposase. Use
these kits for generating DNA sequencing template or truncated cloned
DNA for structure/function studies.
EZ::TN™ Plasmid-Based Deletion Machine DPM9401 10
Reactions
Clone your DNA into a specially constructed plasmid vector. Create sequence
deletions and inversions in the cloned DNA, in vitro, by adding
transposase. The kit includes plasmid cloning vectors, EZ::TN Transposase,
buffer, unlabeled primers for sequencing or mapping, and control DNA.
pWEB::TNC™ Deletion Cosmid Cloning Kit WEBC931 10
Cloning Reactions
Clone your DNA into the specially constructed cosmid cloning vector
for subsequent generation of sequence deletion using the pWEB::TNC Deletion
Cosmid Transposition Kit. The kit includes cosmid cloning vector, buffers,
DNA purification reagents, lambda packaging extracts, and E. coli plating
strains.
pWEB::TNC™ Deletion Cosmid Transposition Kit WEBC942 10
Reactions
Generate sequence deletions and inversions of your cosmid clone prepared
with the pWEB::TNC Deletion Cosmid Cloning Kit. The kit includes EZ::TN
Transposase, buffer, unlabeled primer for sequencing or mapping, and
control DNA.
EZ::TN™ Transposase TNP92110 10
Units
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