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End-Point PCR
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Poster from Plant and Animal Genome Conference 2009 (748k PDF)
Applications
- PCR of challenging plant genomic DNA preparations that contain polyphenols
or other inhibitors.
- PCR of high-GC templates.
PCR amplification from plants such as sunflower and cotton is challenging
due to the presence of polysaccharides, secondary metabolites, and polyphenolic
compounds. The presence of polyphenols also makes it difficult to detect the
DNA of spoilage bacteria in wine samples. The PlantAmp™ PCR System is
formulated to optimize PCR amplification from samples containing polyphenols,
as well as GC-rich templates. No organic solvents or detergent lysis reagents
are required when purifying plant DNA for PCR. We recommend using the QuickExtract™ Plant or Seed DNA
Extraction Solutions for fast, reliable PCR results with the PlantAmp PCR System.
Plants tested include cotton, grape leaf, sunflower, tomato, and grains including
barley, maize, oats, rice, rye, and wheat.
Benefits
- No organic solvents or detergents required for DNA extraction.
- High-fidelity PCR enzyme blend with proofreading activity.
- Optimal PCR results from challenging samples.
- Convenient PreMix format, ideal for high-throughput applications.
"Your QuickExtract Seed and PlantAmp helped me resurrect some DNA
templates that I was on the verge of abandoning due to lack of quality DNA
and subsequent PCR amplification for downstream sequencing."
Millie Burrell
Texas A&M University
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Figure 1. Consistent sunflower-leaf DNA amplifications with the
PlantAmp™ System. A single-copy STR region was amplified
from sunflower-leaf DNA isolated using the QuickExtract™ Seed
Solution. Each amplicon (lanes 2-7) was obtained from a separate leaf
punch. Lane 1, 100-bp ladder. |
PlantAmp PCR System: Purchase of this product includes an immunity from
suit under patents specified in the product insert to use only the amount
purchased for the purchaser's own internal research. No other patent rights
(such as 5´ Nuclease Process patent rights) are conveyed expressly,
by implication, or by estoppel. Further information on purchasing licenses
may be obtained by contacting the Director of Licensing, Applied Biosystems,
850 Lincoln Centre Drive, Foster City, California 94404, USA.
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