Figure 2. RT-PCR amplification of a series of different
messages present in skeletal muscle RNA. RNA was extracted from slide-mounted,
FFPE human tissue samples per the protocol and reverse transcribed with
the MMLV Reverse Transcriptase 1st Strand cDNA Synthesis Kit and random
primers. Skeletal muscle cDNA was amplified to produce short amplicons from
a number of different messages. The products were separated on a 3% agarose
gel and were visualized with SYBR® Gold. Lane M, 100-bp DNA ladder;
lane 1, a 116-bp region of RYR1; lane 2, a 162-bp region of ACTA; lane 3,
a 166-bp region of RSP18; lane 4, a 166-bp region of ENO3; lane 5, a 226-bp
region of GAPDH; lane 6, a 232-bp region of OAZ1; lane 7, a 307-bp region
of ACTB; lane 8, a 308-bp region of TNF. |
Figure 3. PCR amplification of five regions of the 15.4-kb
ryanodine receptor 1 (RYR1) cDNA. RNA was extracted as per the protocol
from FFPE slide-mounted human skeletal muscle tissue samples and reverse-transcribed
with the MMLV Reverse Transcriptase 1st Strand cDNA Synthesis Kit and random
primers. The RYR1 PCR products were separated on a 3% agarose gel and were
visualized with SYBR® Gold. Lane M, 100-bp DNA ladder; RYR1 regions
amplified: lane 1, 123-228; lane 2, 2,866-2,987; lane 3, 9,132-9,279; lane
4, 11,842-11,979; lane 5, 14,935-15,097. |
