PCR |
End-Point PCR
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Applications
- Simplified PCR optimization.
- PCR of difficult or high-GC templates (Fig. 1).
- Multiplex PCR (Fig. 2).
- PCR amplifications up to 20 kb for cloning.
The FailSafe™ PCR System provides dependable, consistent high-fidelity
PCR results for every DNA template, regardless of its source or sequence.
The FailSafe PCR System will faithfully amplify your template every time.
Using EPICENTRE's patented PCR Enhancement Technology, the FailSafe
PCR PreMix Selection Kit has replaced the need for cumbersome adjustments
of reaction components to determine the optimal conditions for PCR of
each template and primer pair. The 12 FailSafe PCR PreMixes in this kit
cover a meticulously determined matrix of PCR conditions that give optimal
PCR results for different sequences. Each PreMix represents a unique
PCR condition, with everything needed for successful PCR. Combine the
template and primers with the FailSafe Enzyme Mix, add one volume of
this cocktail to one volume of each of the 12 FailSafe PCR 2X PreMixes,
and cycle. EPICENTRE guarantees that at least one FailSafe PCR PreMix
will provide conditions that are optimal for the template and primers
tested.
For reliable and consistent PCR results for all subsequent amplifications
using the same primers, simply order the FailSafe PCR System with PreMix
Choice, and specify the same FailSafe PCR PreMix that gave optimal PCR
results using the FailSafe PreMix Selection Kit.
Benefits
- Successful PCR, the first time and every time.
- High PCR accuracy, using a unique PCR Enzyme Mix with a 3-fold lower
error rate than Taq DNA polymerase.
- Extremely high sensitivity and specificity using the PCR Enhancer
Technology.
- FailSafe PCR PreMixes contain all components needed for successful
PCR.
- Generates PCR products suitable for both TA cloning and blunt-end
cloning.
"EPICENTRE products are great, as for FailSafe™ PCR,
we use it all the time."
Prof. Sir Martin Evans, FRS
Nobel Laureate, Cardiff University, Wales, UK
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| Figure 1. Amplification of an 80%-85% GC-rich region of the human
fragile X gene. PCR was performed using the FailSafe™ PCR System,
schematically depicted above. Lanes A-L show the amplification products
resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular
weight marker. In this experiment, optimal amplification was obtained
with FailSafe PCR PreMix J. The size of the expected amplicon is indicated
by an arrow. |
Figure 2. Multiplex PCR of the human CFTR gene. The FailSafe™ PCR
System amplified all five exons of the CFTR gene from as little as 1 ng
of human genomic DNA. Lane M, DNA marker; lane 1, negative control; lanes
2-5, multplex PCR from 1, 10, 50, and 100 ng, respectively, of human genomic
DNA. |
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| Figure 3. FailSafe™ PCR of 85+%-GC human fragile X gene. Fragile
X gene was amplified from as little as 1 ng of total human genomic DNA. |
Figure 4. Detection of the cII gene in one copy of the phage lambda
DNA genome. |
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Figure 5. FailSafe™ PCR of a range of different sequences and
sequence sizes in phage, human and bacterial DNA. PCR products shown
are up to 20 kb for lambda DNA, up to 21.5 kb for human DNA, and up to
18 kb for E. coli DNA. |
FailSafe™ PCR Premix Selection Kits: Purchase of this product includes
an immunity from suit under patents specified in the product insert to use
only the amount purchased for the purchaser's own internal research. No other
patent rights (such as 5´ Nuclease Process patent rights) are conveyed
expressly, by implication, or by estoppel. Further information on purchasing
licenses may be obtained by contacting the Director of Licensing, Applied
Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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