In Vitro Transcription and RNA Research |
In Vitro Transcription Kits
Order
| Protocol
A P266L T7 RNA Polymerase Transcription Kit reaction produces high specific-activity radiolabeled-RNA probes (labeled-NTP not included). The P266L RNA Polymerase contained in the kit is a mutant form of phage T7 RNA Polymerase1. The P266L mutation markedly decreases abortive transcription initiation events - and concomitant production of short 5- to 8-nucleotide abortive RNA transcripts - that can occur with the wild-type T7 RNA polymerase.
Application
- Preparation of high specific-activity RNA probes, especially short RNA probes where contamination by abortive RNA transcripts can cause purification and specificity problems.
Benefits
- Fewer small, abortive RNA transcripts contaminating the desired full-length RNA probe.
- More labeled-NTP incorporated into the desired full-length RNA probe.
- Easier clean-up and less contamination, especially with short, full-length RNA probes.
References
- Guillerez, J., et al., (2005) Proc. Nat'l Acad. Sci. 102 (17), 5958 – 5963.
[top of page]
|
