Unit Definition: One Molecular Biology
Unit (MBU) of Baseline-ZERO™ DNase produces an increase in the
A260 of a solution of dsDNA, of 0.001 per minute at 25°C.
Functionally, 1 MBU completely digests 1 µg of linear pUC19 DNA
to mononucleotides in 10 minutes at 37°C.
Storage Buffer: Baseline-ZERO DNase is supplied in a 50% glycerol
solution containing 50 mM Tris-HCl (pH 7.5), 10 mM CaCl2,
10 mM MgCl2 and 0.1% Triton® X-100.
10X Baseline-ZERO™ DNase Reaction Buffer: 100 mM Tris HCl
(pH 7.5), 25 mM MgCl2 and 5 mM CaCl2.
10X Baseline-ZERO™ DNase Stop Solution: 30 mM EDTA.
Quality Control: Baseline-ZERO DNase is assayed for its ability
to completely digest linear dsDNA to mononucleotides under standard assay
conditions. Baseline-ZERO DNase is free of detectable RNase activities
as assayed by PAGE analysis of 1 µg of a synthetic RNA transcript
following an overnight incubation with sufficient DNase I to completely
digest 1,000 µg of DNA.
References
- Kienzle, N. et al. (1996) BioTechniques 20,
612.
*Patent pending. |
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Figure 2. The use of Baseline-ZERO™ DNase to remove small oligonucleotides
during DNase treatment. Lane M, Kilobase ladder; lanes 1-4, 160 ng
of EcoR I-digested plasmid DNA incubated for 15 minutes at 37°C
as follows: lane 1, untreated; lane 2, incubated with DNase I; lane 3,
with supplier A's hyperactive DNase; lane 4, with Baseline-ZERO DNase.
Only Baseline-ZERO DNase removes the small residual oligos at the bottom
of the gel. |
