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dNTP Solutions
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Sterile, neutral pH solutions of dATP, dCTP, dGTP, and dTTP are available either
as individual nucleotides or a mixture of all four nucleotides for use in DNA
synthesis. Solutions containing only one dNTP are available in 100 mM concentrations.
Premixed solutions containing all four dNTPs are also available at two concentrations,
2.5 mM and 25 mM of each dNTP. Each solution is sufficient for at least 1,000
50-µl reactions at concentrations used in typical reactions with most
DNA polymerases. A sterile, pH neutralized 20 mM solution of dUTP is also available
for synthesis of uracil-containing DNA.
Storage Buffer: dNTP solutions are made with sterile deionized water
and adjusted to pH 7.0.
Quality Control: All of EPICENTRE's dNTP solutions meet spectrophotometric
criteria and are function-tested using our DNA polymerases.
dATP (2´-Deoxyadenosine-5´-Triphosphate)
Formula (free acid): C10H16N5O12P3
Molecular weight: 491
A250/A260 = 0.80
A280/A260 = 0.15 |
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dCTP (2´-Deoxycytidine-5´-Triphosphate)
Formula (free acid): C9H16N3O13P3
Molecular weight: 467
A250/A260 = 0.84
A280/A260 = 0.98 |
dGTP (2´-Deoxyguanosine-5´-Triphosphate)
Formula (free acid): C10H16N5O13P3
Molecular weight: 507
A250/A260 = 1.15
A280/A260 = 0.66 |
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dTTP (2´-Deoxythymidine-5´-Triphosphate)
Formula (free acid): C10H17N2O14P3
Formula weight: 482
A250/A260 = 0.65
A280/A260 = 0.71 |
dUTP (2´-Deoxyuridine-5´-Triphosphate)
Formula (free acid): C9H15N2O14P3
Formula weight: 468
A250/A260 = 0.75
A280/A260 = 0.35 |
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Solutions of 2´-Fluorine-2´-deoxycytidine-5´-triphosphate
(2´-Fluorine-dCTP) and 2´-Fluorine-2´-deoxyuridine- 5´-triphosphate
(2´-Fluorine-dUTP) are also available.
Ribonucleoside-5´-Triphosphate Solutions
2´-Fluorine-2´-Deoxyribonucleoside-5´-Triphosphate Solutions
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