Applications
- Rapid, maximum-yield synthesis of RNA for studies of RNA structure,
processing, ribozymes, RNAi, or antisense RNA.
The AmpliScribe™ T7-Flash™ Transcription Kit uses
EPICENTRE's rapid, high-yield in vitro transcription technology.
An AmpliScribe T7-Flash Transcription reaction yields more RNA
(180 µg per 20-µl reaction) in 30 minutes than other high-yield in
vitro transcription kit reactions produce in 2 hours (Fig. 1), including
EPICENTRE's own AmpliScribe T7 High Yield Kit. The AmpliScribe T7-Flash Kit
utilizes proprietary enzyme formulations that enable the maximum possible
yields of RNA from virtually any template that is downstream of the T7
RNA polymerase promoter, including sequences in linearized plasmids,
cDNA, double-stranded oligos, or PCR products.
Benefits
- Exceptionally high yields of full-length RNA. Reactions can be scaled
up to obtain grams (or more) of RNA.
- When transcribing 1 µg of 1-kb linearized plasmid DNA template,
a standard AmpliScribe T7-Flash reaction is complete in 30 minutes
and yields up to 180 µg of RNA.
- RNase inhibitor included.
- Can be used for "Eberwine"-type RNA amplification procedures.
However, we recommend TargetAmp™ 2-Round aRNA Amplification Kits
for amplification of mRNA from laser-capture microdissection or other
small samples.
|
Figure 1. An AmpliScribe™ T7-Flash™ transcription
reaction is complete in 30 minutes and yields more RNA than other 2-hour "high-yield" transcription
reactions. Yields shown were obtained using 1 µg of a linear
DNA template in a 20-µl reaction, producing a 1.4-kb RNA. |
