Transposomics™ Research |
In Vivo Transposomics™ Research
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Applications
- In vitro insertion of an artificial Mu transposon into DNA cloned
in vectors, such as plasmids, fosmids, cosmids, or BACs, as well as in
vitro insertion into linear DNA.
- Preparation of HyperMu Transposomes for in vivo transposition* of
an artifical Mu transposon following electroporation into living cells.
HyperMu™ MuA Transposase is a hyperactive enzyme that is at least 50
times more active than native MuA transposase available from other suppliers
of Mu-based systems. The highly purified, single-subunit HyperMu MuA Transposase
can be used to randomly insert (transpose or "hop") any HyperMu™ Transposon
into any target DNA in vitro with an efficiency of up to >106 insertion
clones per standard reaction. HyperMu Transposase can also catalyze transposition
of other artificial Mu transposons that have the R1 and R2 recognition sequences
in the proper orientation at each end. The DNA between the R1 and R2 transposon
end sequences can be any DNA, including DNA encoding selectable markers, origins
of replication, or other genetic elements.
Further, when HyperMu MuA Transposase is incubated with an artificial Mu transposon
in the absence of Mg2+, a stable HyperMu Transposome complex is
formed. Like an EZ-Tn5™ Transposome™ complex, a HyperMu Transposome
can be electroporated into living cells, where it is activated by intracellular
Mg2+ and the Mu transposon component is randomly inserted into the
host's DNA.
A typical HyperMu transposition reaction requires four components: 1) the
HyperMu Transposase; 2) an artificial Mu transposon; 3) a target DNA; and 4)
the presence of Mg2+. Insertion of an artificial Mu transposon into
the target DNA results in a 5-bp duplication of target DNA sequence immediately
adjacent to both ends of the transposon.
Benefits
- At least 50 times more active than the MuA transposase available from other
suppliers.
- HyperMu MuA Transposase recognizes the same R1 and R2 transposon recognition
sequences as MuA transposase and is therefore compatible with other artificial
Mu transposons.
*The use of Transposome™ complexes for in vivo insertion of
a transposon, including, but not limited, to HyperMu™ and EZ-Tn5™ Transposome™ complexes,
is covered by U.S. Patent No. 6,159,736 and related patents and patent applications,
exclusively licensed to EPICENTRE.
HyperMu™ <KAN-1> and <CHL-1> Insertion Kits
HyperMu™ <R6Kγori/KAN-1>Tnp Transposome™ Kit
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