Cloning and Library Construction |
Screening of Recombinants
Order
| Protocol
| Citations
| Related Products
Applications
- Identification of desired clones in a library.
- Determining the orientation of cloned inserts.
- PCR of bacterial genomic DNA.
The Colony Fast-Screen™ Kit (PCR Screen) provides a rapid method for
preparing clones for screening by PCR. Using the Colony Fast-Screen Kit (PCR
Screen) there is no need to grow cultures or purify DNA prior to PCR (Fig. 1).
The kit can be used with all standard E. coli hosts and
all cloning vectors. Thermostable polymerase and PCR primers are not provided.
Benefits
- Identify desired clones by PCR without the need to grow cultures or purify
DNA (Fig. 2).
- Enables PCR screening of high-copy clones, single-copy clones, and bacterial
genomic DNA.
- Amenable to high-throughput applications.
- DNA preparation process does not interfere with pre-established PCR conditions.
- Enables both long and short PCR amplifications and can be used with all
standard E. coli host strains.
 |
 |
| Figure 1. Schematic overview of the protocol for the Colony
Fast- Screen™ Kit (PCR Screen). |
Figure 2. The Colony Fast-Screen™ Kit (PCR Screen)
enables screening of both high-copy and single-copy clones by PCR without
the need for cultures and DNA purifications. Colonies containing a 5-kb
lambda fragment cloned into the CopyControl™ pCC1™ vector and
grown on plates at either single-copy or high-copy number were picked and
processed using the Colony Fast-Screen Kit (PCR Screen). PCR was performed
using primers homologous to the ends of the cloning vector. Lane 1, DNA size
marker; lane 2, E. coli DNA (negative control); lane 3, single-copy
clone; lane 4, high-copy clone; lane 5, PCR of 1 ng of purified DNA. |
Colony Fast-Screen™ Kit (Restriction Screen)
Colony Fast-Screen™ Kit (Size Screen)
Fast-Link™ DNA Ligation Kit
TransforMax™ EC100™ Electrocompetent E. coli
TransforMax™ EC100™ Chemically Competent E. coli
TransforMax™ EC100™-T1R Electrocompetent E. coli
TransforMax™ EPI300™ Electrocompetent E. coli
TransforMax™ EPI300™ Chemically Competent E. coli
[top of page]
|
