Cloning and Library Construction |
Competent Cells, Transformation, and Packaging
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Applications
- Routine cloning of DNA from plasmid, fosmid, and BAC vectors, with resistance
to phage T1 and T5.
Phage T1-resistant TransforMax™ EC100™-T1R E. coli have
all the benefits of the highly versatile TransforMax EC100™ E. coli competent
cells with the addition of being resistant to bacteriophages T1 and T5 (tonA genotype).
Phage T1-resistant TransforMax EC100-T1R E. coli are ideal
for most cloning applications. However, the phage T1-resistant TransforMax EC100-T1R E.
coli should not be used with EPICENTRE's CopyControl™ Cloning Systems.
Benefits
- tonA for resistance to phage T1 and phage T5.
- High transformation efficiency with clones of all sizes–including
BAC clones.
- lacZΔM15 for blue/white screening of recombinants.
- Readily accepts large DNAs for construction of large-insert genomic libraries.
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient
cloning of methylated DNA.
- Endonuclease minus (endA1) to ensure high yields of DNA.
- Recombination minus (recA1) for greater stability of large cloned
inserts.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74
recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL
(StrR) nupG tonA
Electroporation Cuvettes
EpiFOS™ Fosmid Library Production Kit
Fast-Link™ DNA Ligation Kit
pWEB-TNC™ Cosmid Cloning Kit
pWEB™ Cosmid Cloning Kit
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