Applications
- Size analysis of BAC clones by agarose minigel electrophoresis.
- Size analysis of supercoiled plasmid DNA or extrachromosomal DNA from
any source.
The BAC-Tracker™ Supercoiled DNA Ladder is designed for estimating
the size of large supercoiled DNAs, such as Bacterial Artificial Chromosome
(BAC) clones, by agarose gel electrophoresis. The BAC-Tracker Ladder contains
seven discrete supercoiled DNAs ranging from 8 kb to 165 kb (Fig. 1). The
8-kb band facilitates screening for "empty" or nonrecombinant BAC
clones.
The BAC-Tracker Supercoiled DNA Ladder is provided in a ready-to-load solution.
Simply load 10 µl of BAC-Tracker Supercoiled DNA Ladder per gel lane.
Run the gel, and stain using SYBR® Gold. The BAC-Tracker Ladder can
be used with the Colony Fast-Screen™ Kit (Size Screen) to estimate
the size of BAC clones in less than 4 hours.
Quality Control: Each batch of BAC-Tracker Supercoiled DNA Ladder
is checked by gel electrophoresis to ensure the correct size of all seven
supercoiled DNA bands. |
 |
Figure 1. Supercoiled BAC DNA is sized by comparison with the BAC-Tracker™ Supercoiled
DNA Ladder. Colonies from three different BAC clones (lanes 1-3) were
processed using the Colony Fast-Screen™ Kit (Size Screen) and analyzed
on a 1% agarose gel. Lane M, BAC-Tracker Supercoiled DNA Ladder. |
