Applications
- Single-strand conformation polymorphism (SSCP) analysis of PCR products.1,2
- Generation of ssDNA sequencing template from PCR products.
Lambda Exonuclease is a highly processive 5´→3´ exodeoxyribonuclease
that selectively digests the 5´ phosphorylated strand of dsDNA.
The preferred substrate is blunt-ended, 5´-phosphorylated dsDNA.
The enzyme has reduced activity against nicked DNA and against ssDNA
and gapped DNA.3
Unit Definition: One unit of Lambda Exonuclease will produce
10 nmol of acid-soluble deoxyribonucleotides from dsDNA template in 30
minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer.
Quality Control: Lambda Exonuclease is function-tested to ensure
complete and preferential degradation of PCR products produced using
5´-phosphorylated primers. PCR products made using primers containing
5´-OH ends are not digested. Lambda Exonuclease is tested to be
free of contaminating endonuclease activities.
References
- Schwieger, F. and Tebbe, C.C. (1998) App. and Environ. Microb. 64,
4870.
- Schwieger, F. and Tebbe, C.C. (2000) App. and Environ. Microb. 66,
3556.
- Mitsis, P.G. and Kwagh, J.G. (1999) Nucleic Acids Res. 27,
3057.
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