Applications
- Routine cloning of DNA up to 200 kb.
The highly versatile TransforMax™ EC100™ E. coli competent
cells are ideal for most cloning applications. The cells provide very high
transformation efficiency when tested against a wide range of supercoiled
DNAs as well as DNA directly from a ligation reaction (Table 1).
Benefits
- High transformation efficiency with clones of all sizes, including BAC
clones (Table 1).
- lacZΔM15 for blue/white screening of recombinants.
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient
cloning of methylated DNA.
- Endonuclease minus (endA1) to ensure high yields of DNA.
- Recombination minus (recA1) for greater stability of large cloned
inserts.
|
| pUC19 |
1.4 x 108 |
1.4 x 1010 |
| 8.1-kb Clone |
1.3 x 107 |
Not tested |
| 13.1-kb Clone |
4.3 x 106 |
1.3 x 109 |
| 23.1-kb Clone |
9.2 x 105 |
3.0 x 108 |
| 145-kb BAC Clone |
Not tested |
7 x 107 |
| 13.1-kb clone directly from a ligation reaction |
2.2 x 105 |
2.1 x 107 |
Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with
a variety of DNAs. Transformations were performed using
50 µl of competent cells and either supercoiled DNAs of the indicated
sizes or a 1-µl aliquot from a standard 10-µl ligation reaction.
Results shown are in cfu/µg of DNA and are the average transformation
efficiencies obtained from several trials. |
