EPICENTRE Biotechnologies
EPICENTRE Biotechnologies  
  Log In
In Vitro Transcription and RNA Research | RNA Polymerases and Transcription Research

T7, T3, and SP6 RNA Polymerases

Order | Protocol | Citations | Related Products

Applications

  • Synthesis of RNA for nucleic acid amplification methods or gene expression studies.
  • RNA synthesized can be used as: a hybridization probe, antisense RNA, a ribozyme, a template for in vitro translation, a precursor mRNA for splicing or other processing studies, or to make dsRNA for RNA interference or gene silencing.

T7, T3, and SP6 RNA Polymerases produce defined RNA by in vitro transcription of dsDNA that is downstream of the specific RNA polymerase promoter. These enzyme preparations are highly purified, and exhibit excellent promoter specificity.

Notes:

  1. A buffer package containing 5X Transcription Buffer and DTT may be purchased separately.
  2. EPICENTRE also offers in vitro transcription kits that are optimized and pre-tested for a variety of applications.
    See the In Vitro Transcription Product Selection Guide to find the best kit for your application.

Unit Definition: One unit of RNA polymerase catalyzes the incorporation of 1 nmol of ribonucleoside triphosphate into RNA in 1 hour at 37°C under standard assay conditions using a DNA template with the appropriate T7, SP6, or T3 promoter.

Storage Buffer: 50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1.0 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.

5X Transcription Buffer (available separately): 0.2 M Tris-HCl (pH 7.5), 50 mM NaCl, 30 mM MgCl2, and 10 mM spermidine.

Quality Control: RNA polymerases are tested in in vitro transcription reactions, and are free of detectable exo- and endonuclease and RNase activities, and E. coli RNA polymerase activity.

References

T7 RNA Polymerase

  1. Davanloo, P. et al. (1984) Proc. Natl. Acad. Sci. USA 81, 2035.
  2. Eberwine, J. (1992) Proc. Natl. Acad. Sci. USA 89, 3010.(Note: Use of T7 RNA Polymerase for this application may require a license.)
  3. Stahl, S. J. and Zinn, K. (1981) J. Mol. Biol. 148, 481.

T3 RNA Polymerase

  1. Konarska, M.M. et al. (1984) Cell 38, 731.
  2. Parvin, J.D. et al. (1986) DNA 5, 167.

SP6 RNA Polymerase

  1. Butler, E.T. and Chamberlin, M.J. (1982) J. Biol. Chem. 257, 5772.
  2. Melton, D.A. et al. (1984) Nucleic Acids Res. 12, 7035.

To view ordering information, choose your country of origin.
If your country is not listed, contact us.

   Catalog No. Concentration Size
T7 RNA Polymerase
   T7905K 50 U/µl 5,000 Units
   T7950K 50 U/µl 50,000 Units
   TM910K 200 U/µl 10,000 Units
   TH950K 1,000 U/µl 50,000 Units
   TU950K 2,500 U/µl 50,000 Units
(Enzyme only. Transcription Buffer is not included.)
T3 RNA Polymerase
   T4905K 50 U/µl 5,000 Units
   T9050K 50 U/µl 50,000 Units
(Enzyme only. Transcription Buffer is not included.)
SP6 RNA Polymerase
   S4905K 50 U/µl 5,000 Units
   S4950K 50 U/µl 50,000 Units
   SM910K 200 U/µl 10,000 Units
(Enzyme only. Transcription Buffer is not included.)
Transcription Buffer Package
   BP1001 1 Pkg
Includes 5 ml of 5X Transcription Buffer and 2.5 ml of 100 mM DTT.

You may wish to consider the following related products:

AmpliScribe™ T7, T3, and SP6 High Yield Transcription Kits
AmpliScribe™ T7-Flash™ Transcription Kit
Baseline-ZERO™ DNase
Ribonucleoside-5´-Triphosphate Solutions
2´-Fluorine-2´-Deoxyribonucleoside-5´-Triphosphate Solutions
RNA Cap Analogs

[top of page]


HOME | PRODUCTS | WHAT'S NEW | ORDERING | TECHNICAL RESOURCES | CONTACT US | ABOUT US

Bookmark and Share

EPICENTRE® Biotechnologies - Understanding the Genome and Transcriptome
©2010 EPICENTRE Biotechnologies. All Rights Reserved.