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Cloning and Library Construction| Competent Cells, Transformation, and Packaging

MaxPlax™ Lambda Packaging Extracts

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Applications

  • Packaging of lambda, cosmid, or fosmid DNA for construction of genomic libraries.

MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging efficiencies of cos site-containing methylated or unmethylated DNA. Traditional lambda packaging extracts are derived from two complementary lysogenic E. coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax Extracts use a restriction-free E. coli K-12 strain, NM759, in place of strain BHB2690.2 The sonicated extracts of NM759 are combined with extracts of BHB2688 to produce MaxPlax Extracts.

Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging efficiency of greater than 1 x 109 pfu/µg of Control Ligated Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided with each lot of MaxPlax Extracts.

Benefits

  • Packaging efficiencies of up to 3 x 109 pfu/µg DNA.
  • MaxPlax Extracts are devoid of all known restriction activities. Packages highly methylated DNA as efficiently as unmethylated DNA.3
  • No premixing of different extract components required before use.
Table 1. Packaging of methylated and unmethylated DNA using MaxPlax™ Lambda Packaging Extracts.
Cosmid Insert Pfu/µg DNA
Unmethylated DNA 3.5 x 107
Methylated DNA 3.9 x 107

References

  1. Hohn, E.G. (1979) Methods Enzymol. 68, 299.
  2. Gunther, E.G. et al. (1993) Nucleic Acids Res. 21, 3903.
  3. Fiandt, M. (2000) EPICENTRE Forum 7(3), 14.

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   Catalog No. Size
MaxPlax™ Lambda Packaging Extracts
   MP5105 5 Extracts
   MP5110 10 Extracts
   MP5120 20 Extracts
Contents: Predispensed MaxPlax™ Extracts, Control Lambda DNA, Control Host Cells (E. coli).

You may wish to consider the following related products:

CopyControl™ Fosmid Library Production Kit
CopyControl™ HTP Fosmid Library Production Kit
EpiFOS™ Fosmid Library Production Kit
pWEB-TNC™ Cosmid Cloning Kit
pWEB™ Cosmid Cloning Kit

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