Cloning and Library Construction |
Competent Cells, Transformation, and Packaging
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Applications
- Packaging of lambda, cosmid, or fosmid DNA for construction of genomic libraries.
MaxPlax™ Lambda Packaging Extracts offer maximum in vitro packaging
efficiencies of cos site-containing methylated or unmethylated DNA. Traditional
lambda packaging extracts are derived from two complementary lysogenic E.
coli strains, BHB2690 and BHB2688, as described by Hohn.1 MaxPlax
Extracts use a restriction-free E. coli K-12 strain, NM759, in place
of strain BHB2690.2 The sonicated extracts of NM759 are combined
with extracts of BHB2688 to produce MaxPlax Extracts.
Each lot of MaxPlax Extracts is tested and guaranteed to maintain a packaging
efficiency of greater than 1 x 109 pfu/µg of Control Ligated
Lambda DNA for 1 year from the date of purchase if stored properly. A Certificate
of Analysis stating actual packaging efficiency (pfu/µg DNA) is provided
with each lot of MaxPlax Extracts.
Benefits
- Packaging efficiencies of up to 3 x 109 pfu/µg DNA.
- MaxPlax Extracts are devoid of all known restriction activities. Packages
highly methylated DNA as efficiently as unmethylated DNA.3
- No premixing of different extract components required before use.
|
| Cosmid Insert |
Pfu/µg DNA |
| Unmethylated DNA |
3.5 x 107 |
| Methylated DNA |
3.9 x 107 |
|
References
- Hohn, E.G. (1979) Methods Enzymol. 68, 299.
- Gunther, E.G. et al. (1993) Nucleic Acids Res. 21,
3903.
- Fiandt, M. (2000) EPICENTRE Forum 7(3), 14.
CopyControl™ Fosmid Library Production Kit
CopyControl™ HTP Fosmid Library Production Kit
EpiFOS™ Fosmid Library Production Kit
pWEB-TNC™ Cosmid Cloning Kit
pWEB™ Cosmid Cloning Kit
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