MasterAmp™ PCR Optimization Kits

• Simplified PCR optimization with a thermostable DNA polymerase of choice.
• PCR of difficult or high-GC templates.
• PCR amplifications up to 20 kb.
• Multiplex PCR.

For rapid, dependable optimization of PCR, we encourage you to try the FailSafe™ PCR System to obtain the best PCR results with any template up to ~20 kb. However, if you prefer to use another enzyme, EPICENTRE also has two MasterAmp™ PCR Optimization Kits that will help you to obtain the best possible PCR results with thermostable enzymes frequently used in PCR, such as Taq, Pfu, Tth, Tfl, and AmpliTherm™ DNA polymerases. These MasterAmp PCR Optimization Kits contain a series of 12 MasterAmp PCR 2X PreMixes that have been meticulously tested and represent a complete range of PCR conditions frequently encountered. Each MasterAmp 2X PreMix contains all components needed for PCR except for the primers, the template, and the PCR enzyme.

First, perform PCR with your template, primers, and the 12 MasterAmp PreMixes. Select the PreMix that gives optimal results. Then, use the same MasterAmp PCR PreMix to obtain reliable, consistent PCR of the same sequence.

Benefits

• Fast, simple optimization.
• Extremely high sensitivity and specificity using the PCR Enhancer Technology.
• MasterAmp PCR PreMixes contain all components needed for successful PCR.
• Generates PCR products suitable for both TA cloning and blunt-end cloning.

A. A 75%-GC-rich ApoE gene region was amplified in total human genomic DNA using PreMixes A-L. MasterAmp PCR PreMix K produced optimal results in Step 1.