Sample Preparation |
Rapid Extraction of PCR-Ready DNA and RNA
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Applications
- Rapid, easy extraction of DNA from buccal swab samples for human or animal
identity testing, or SNP analysis.
- PCR-based assays for viruses, bacteria, or other microorganisms.
The MasterAmp™ Buccal Swab DNA Extraction Kits are easy-to-use, single-tube
systems for the rapid preparation of DNA from human or other mammalian buccal
(cheek) cells for PCR assays. The format allows processing of one to hundreds
of samples in less than an hour. Yields range from 0.5 to 3.0 µg of DNA
per sample, enough to perform several PCR amplifications. The kits include sterile
Buccal Swab Brushes, aliquots of DNA Extraction Solution, and complete protocols
for isolation of DNA from buccal samples.
Benefits
- Quick and easy procedure.
- Reduces risks from sample collection (e.g., needle sticks) and uses no phenol,
chloroform, or other toxic solvents.
- Suitable for high-throughput and automated assays.
- DNA yields of 0.5-3.0 µg per buccal sample.
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Figure 1. Multiplex amplification of DNA purified using the MasterAmp™ Buccal
Swab DNA Extraction Kit. Panel A, human genomic DNA was purified from
four individuals using the MasterAmp Buccal Swab Kit and the DNA was separated
on a 1% agarose gel. Panel B, multiplex amplification of five sets of CHLC
markers was performed and the samples were resolved on a 3% agarose gel.
Lanes 1, 3, 5, 7, 100-bp ladder; Lanes 2, 4, 6, 8, multiplex PCR of the
isolated DNA shown in Lanes 1, 2, 3, and 4 (Panel A), respectively. |
BuccalAmp™ DNA Extraction Kit
FailSafe™ GREEN and PROBES, TAQurate™ GREEN and PROBES, MasterAmp™ GREEN, TAQXpedite™ GREEN (discontinued)
FailSafe™ PCR System
MasterAmp™ DNA Polymerases
TAQXpedite™ PCR System (FAST End-Point)
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