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PCR| End-Point PCR

FailSafe™ PCR System

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Applications

  • Simplified PCR optimization.
  • PCR of difficult or high-GC templates (Fig. 1).
  • Multiplex PCR (Fig. 2).
  • PCR amplifications up to 20 kb for cloning.

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time.

Using EPICENTRE's patented PCR Enhancement Technology, the FailSafe PCR PreMix Selection Kit has replaced the need for cumbersome adjustments of reaction components to determine the optimal conditions for PCR of each template and primer pair. The 12 FailSafe PCR PreMixes in this kit cover a meticulously determined matrix of PCR conditions that give optimal PCR results for different sequences. Each PreMix represents a unique PCR condition, with everything needed for successful PCR. Combine the template and primers with the FailSafe Enzyme Mix, add one volume of this cocktail to one volume of each of the 12 FailSafe PCR 2X PreMixes, and cycle. EPICENTRE guarantees that at least one FailSafe PCR PreMix will provide conditions that are optimal for the template and primers tested.

For reliable and consistent PCR results for all subsequent amplifications using the same primers, simply order the FailSafe PCR System with PreMix Choice, and specify the same FailSafe PCR PreMix that gave optimal PCR results using the FailSafe PreMix Selection Kit.

Benefits

  • Successful PCR, the first time and every time.
  • High PCR accuracy, using a unique PCR Enzyme Mix with a 3-fold lower error rate than Taq DNA polymerase.
  • Extremely high sensitivity and specificity using the PCR Enhancer Technology.
  • FailSafe PCR PreMixes contain all components needed for successful PCR.
  • Generates PCR products suitable for both TA cloning and blunt-end cloning.
 Never Fail...With FailSafe™ PCR Systems
Figure 1 Figure 2
Figure 1. Amplification of an 80%-85% GC-rich region of the human fragile X gene. PCR was performed using the FailSafe™ PCR System, schematically depicted above. Lanes A-L show the amplification products resulting from PCR using the 12 FailSafe PCR PreMixes. Lane M, molecular weight marker. In this experiment, optimal amplification was obtained with FailSafe PCR PreMix J. The size of the expected amplicon is indicated by an arrow. Figure 2. Multiplex PCR of the human CFTR gene. The FailSafe™ PCR System amplified all five exons of the CFTR gene from as little as 1 ng of human genomic DNA. Lane M, DNA marker; lane 1, negative control; lanes 2-5, multplex PCR from 1, 10, 50, and 100 ng, respectively, of human genomic DNA.
Figure 3 Figure 4
Figure 3. FailSafe™ PCR of 85+%-GC human fragile X gene. Fragile X gene was amplified from as little as 1 ng of total human genomic DNA. Figure 4. Detection of the cII gene in one copy of the phage lambda DNA genome.
Figure 5 Figure 5. FailSafe™ PCR of a range of different sequences and sequence sizes in phage, human and bacterial DNA. PCR products shown are up to 20 kb for lambda DNA, up to 21.5 kb for human DNA, and up to 18 kb for E. coli DNA.

FailSafe™ PCR Premix Selection Kits: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.


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   Catalog No. Size
FailSafe™ PCR PreMix Selection Kit (all 12 Premixes)
   FS99060 60 Units
Includes FailSafe™ PCR Enzyme Mix and all 12 FailSafe™ PCR 2X PreMixes.
FailSafe™ PCR System with PreMix Choice (any one Premix)
   FS99100 100 Units
Includes FailSafe™ PCR Enzyme Mix and choice of any one FailSafe™ PCR 2X PreMix (see below).
FailSafe™ PCR System with PreMix Choice (any two Premixes)
   FS99250 250 Units
Includes FailSafe™ PCR Enzyme Mix and choice of any two FailSafe™ PCR 2X PreMixes (see below).
FailSafe™ PCR System with PreMix Choice (any eight PreMixes)
   FS9901K 1000 Units
Includes FailSafe™ PCR Enzyme Mix and choice of any eight FailSafe™ PCR 2X PreMixes (see below).
FailSafe™ PCR 2X PreMixes (PCR System inclusion only)
   FSP995A 2.5 ml
   FSP995B 2.5 ml
   FSP995C 2.5 ml
   FSP995D 2.5 ml
   FSP995E 2.5 ml
   FSP995F 2.5 ml
   FSP995G 2.5 ml
   FSP995H 2.5 ml
   FSP995I 2.5 ml
   FSP995J 2.5 ml
   FSP995K 2.5 ml
   FSP995L 2.5 ml
Contents: dNTPs, buffer, and various amounts of MgCl2 and FailSafe™ PCR Enhancer with Betaine.

Note: This listing is only for indicating a choice of PreMixes included with the FailSafe™ PCR System (see above). The PreMixes are also available for purchase separately.
FailSafe™ Enzyme Mix Only
   FSE51100 100 Units
   FSE5101K 1000 Units
Note: We can only guarantee the failsafe nature of this system if the FailSafe™ Enzyme Mix is used with a FailSafe™ PCR 2X PreMix that was first determined using the FailSafe™ PCR PreMix Selection Kit (above).

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