Applications
- Preparation of complete and unbiased fosmid libraries that can be maintained
at single-copy number.
The EpiFOS™ Fosmid Library Production Kit provides all reagents needed
to construct up to 10 complete and unbiased primary fosmid libraries. Fosmid
clones constructed in the pEpiFOS-5 Vector cannot be induced to high-copy
number, limiting the DNA yields during purification. For this reason, EPICENTRE
researchers prefer the use of the CopyControl™ Fosmid Library Construction
Kit in the construction of their genomic libraries.
Primers for end-sequencing pEpiFOS-5 clones are available separately.
Benefits
- EpiFOS Vectors are supplied Cloning-Ready: linearized, dephosphorylated,
purified, and ready for ligation.
- No need for partial restriction endonuclease digests or pulse-field gel
electrophoresis to prepare the genomic DNA for cloning.
- High-efficiency lambda packaging eliminates background and false positives.
- Faster and easier than BAC cloning.
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Figure 2. EpiFOS™-5 Fosmid Vector. |
