Applications
- Repair sheared, nebulized, or restriction enzyme-digested genomic DNA
for: preparation of templates for next-gen sequencing; or shotgun library
preparation.
The End-It™ DNA End-Repair Kit converts DNA containing damaged or
incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated,
blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning
vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA
Ligation Kit.
The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair
reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).
Benefits
- The repaired DNA is blunt-ended and 5´-phosphorylated for immediate
blunt-end ligation.
- The high specific activity of the End-Repair Enzyme Mix provides complete
conversion of protruding ends to 5´-phosphorylated, blunt-ended DNA.
|
 |
