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Enzymes for Molecular Biology | Additional Reagents

10X TA Buffer

Order | Protocol

TA Buffer is compatible with a wide variety of restriction endonucleases and nucleic acid modifying enzymes.1,2 Use of TA Buffer can reduce the number of buffer changes required during nucleic acid sample processing, saving time and eliminating sample loss that commonly occurs with buffer changes. Table 1 shows many enzymes that are active in TA Buffer.

10X TA Buffer: 330 mM Tris-acetate (pH 7.8), 660 mM potassium acetate, 100 mM magnesium acetate, and 5 mM DTT.

Table 1. Restriction enzymes and nucleic acid modifying enzymes active in TA Buffer.

Modifying Enzymes
APex™ Heat-Labile Phosphatase T7 DNA Polymerase
NTPhos™ Phosphatase Exonuclease I
T4 Polynucleotide Kinase Exonuclease III
Fast-Link™ DNA Ligase Lambda Terminase
T4 DNA Ligase RNase-Free DNase I
T4 RNA Ligase Plasmid-Safe™ DNase
T4 DNA Polymerase  
 
Restriction Enzymes
Aat II Dra I Kpn I Pst I Spe I
Apa I EcoR I Mbo I Rsr I Stu I
Avr II EcoR V Msc I Sac I Xba I
BamH I Fsp I Nci I Sac II Xho I
Ban I Hae III Nco I Sau3A I Xmn I
Ban II Hinc II Nde I Sfi I  
Bgl II Hind III Nhe I Sma I  
Cla I Hpa I Pme I Sna I  

References

  1. O'Farrell, P.H. et al. (1980) Molec. Gen. Genet. 179, 421.
  2. O'Farrell, P. (1996) EPICENTRE Forum (32), 5.

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   Catalog No. Size
10X TA Buffer
   TA6115 1.5 ml
   TA6160 6.0 ml

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