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  • DNA and RNA Polymerases
  • Phosphatases, Kinases, & Ligases
  • Nucleases, Glycosylases, & DNA Binding Proteins
  • Transposomics™
  • Glucosyltransferases
• WHAT'S NEW
  • New Products
    • Pvu Rts1I Endonuclease
    • T4 Beta-glucosyltransferase
    • Ribo-Zero™ Magnetic Kit (Human/Mouse/Rat)
    • ScriptSeq™ v2 RNA-Seq Library Preparation Kit
    • Ribo-Zero™ Gold Kit (Human/Mouse/Rat)
    • Ribo-Zero™ rRNA Removal Kit (Meta-Bacteria)
    • Terminal deoxynucleotidyl Transferase, Recombinant
    • EZ-Tn5™ Custom Transposome™ Construction Kits
    • T4 RNA Ligase 2, Deletion Mutant
    • TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®
  • Meetings/Trade Shows
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Epicentre Forum: Index By Issue

Below is a listing of Epicentre Forums in chronological order. Each title links to the full issue (where available) while the article titles link to the individual articles. Most issues require PDF viewing software, such as Adobe Acrobat Reader.

Note: Some products in older articles have been discontinued. Please check current availability.

Volume 16 * Number 3 * 2009 (3 MB)

• Nextera™ Technology for NGS DNA Library Preparation:Simultaneous Fragmentation and Tagging by In Vitro Transposition
• Extraction of Bacterial DNA from Gram-Positive and Gram-Negative Species
• Purification of RNA from Recalcitrant Plant Tissues
• Ask Frank - AmpliScribe™ T7-Flash™ and DuraScribe® In Vitro Transcription Kits
• Product Highlights

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Volume 16 * Number 2 * 2009 (3.28 MB)

• High-Throughput Sequence Analysis of 5´ Ends of Transcripts from Blumeria graminis
• Sensitive Nucleic Acid Detection by Primer Generation-Rolling Circle Amplification
• RNA Amplification and cDNA Synthesis for qRT-PCR Directly from a Single Cell without RNA Purification
• Rapid RNA Extraction from Cultured Cells for RT-PCR
• Ask Frank - ExactSTART™ Platform Technology
• Product Highlights

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Volume 16 * Number 1 * 2009 (2.45 MB)

• Introducing the ExactSTART™ Platform of Transcript Discovery and Analysis Tools
• Small-RNA Transcriptome Analysis: A Rapid and Simple Method for Generating cDNA from End-Tagged RNA
• Rapidly Tag the True 5´ End of Eukaryotic mRNAs with the ExactSTART™ Eukaryotic mRNA 5´- & 3´-RACE Kit
• Rapid DNA Extraction from Plant Seeds for PCR
• Fast Recovery of Plasmid and Fosmid DNA in a 96-Well Plate for High-Throughput Sequencing
• Ask Frank - RNA Purification
• Product Highlights

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Volume 15 * Number 1 * 2008 (3.18 MB)

• Rapid, Easy Extraction of DNA and RNA from Archival Samples
• Direct Isolation of Metagenomic DNA from Environmental Water Samples
• A Sense-RNA Amplification Method that Preserves Both 3'and 5' Transcript Information
• Fast PCR with Long and Difficult Amplicons
• Greatly Improved Real-Time RT-PCR from Small Quantities of Degraded RNA Samples
• Ask Frank - Production and Labeling of Antisense RNA using Epicentre's TargetAmp™ Kits
• Product Highlights

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Volume 14 * Number 2 * 2007 (2.94 MB)

• ExactStart™ Full-Length cDNA Library Cloning Kit: A Rapid and Efficient Method to Synthesize Full-Length cDNA for Cloning and Accurate Mapping of Transcription Initiation and Polyadenylation Sites
• Fast Real-Time PCR with a Standard qPCR Thermocycler!
• Easy and Rapid Extraction of Plant DNA Using the QuickExtract™ Plant DNA Extraction Solution
• Ask Frank - Questions about RNA capping and in vitro transcription
• A Simple Enzymatic Process for Generating mRNA-Enriched Poly(A)-Tailed RNA from Total RNA Samples
• Protein Expression from a Chromosomally-Inserted Transposon
• Isolate Intron Lariat Loops with RNase R
• Talk Tidbits

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Volume 14 * Number 1 * 2007 (2.87 MB)

• The New mScript™ mRNA Production System – Efficient mRNA Transcription, Capping and Tailing for the Highest Yields of Active Protein
• Detect More Transcripts and Use Less RNA in Your Microarray Studies; TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 104
• A Sensitive Method For 5'-Terminal End-group Analysis of RNA Enabled by Terminator™ 5'-Phosphate-Dependent Exonuclease
• Ask Frank
• Synthesis of Full-Length cDNA Greater Than 15 kb for qPCR Using the New MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit
• Rapid Sampling and Identification of Mitochondrial and Genomic DNA
• PCR Analysis of Fingernail DNA Using QuickExtract™ DNA Extraction Solution
• Make Circular ssDNA in 1 Hour Without Using Oligo "Bridges"
• Biotin-16-UTP Now Available
• Prepare Labeled RNA Probes More Efficiently and Economically Using the AmpliScribe™ T7 Aminoallyl-RNA Transcription Kit
• Also Available: Biotin-X-X-NHS

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Volume 13 * Number 4 * 2006 (1.6 MB)

• Achieve 100% Capping Efficiency with the NEW ScriptCap™ m⁷G Capping System
• Improve the Translation Efficiency of any 5'-Capped mRNA with the NEW ScriptCap™ 2'-O-Methyltransferase
• High Sensitivity RT-PCR from as Little as 1 Cell
• Direct Genomic DNA Sequencing for Rapid Fungal Identification
• Ask Frank
• PCR Amplification of a High-GC Region of the Glucocorticoid Receptor Gene Using the FailSafe™ PCR System
• 3'/5' Ratio Analysis of FFPE RNA Purified by the MasterPure™ RNA Purification Kit
• Rapid and Highly Sensitive Screening of Bacterial RNA Polymerase Activity and Inhibitors Using the Kool™ NC-45™ Universal RNA Polymerase Templates
• Obtain Higher Molecular Weight Soil DNA without Bead-Beating

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Volume 13 * Number 3 * 2006 (2.5 MB)

• High-Throughput Isolation of BAC DNA That is Free of E. coli Genomic DNA and RNA
• The EZ-Tn5™ Transposome™ Complex Simplifies Microbial Engineering
• Achieve High Fidelity, High Sensitivity, and High Yield RNA Amplification That Produces High Quality Array Results
• In 30 Minutes Obtain PCR-Ready DNA from FFPE Tissue with QuickExtract™ DNA Extraction Solution
• Ask Frank
• FailSafe™ PROBES Real-Time PCR Optimization Kit Greatly Improves Multiplex Performance
• Extensive Range of Plant Genomic Libraries Made with the CopyControl™ Fosmid Library Production Kit
• Sensitive qRT-PCR Obtained from the Lysates of 1 – 10 Cells Using the MessageBOOSTER™ cDNA Synthesis Kit for qPCR

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Volume 13 * Number 2 * 2006 (1.6 MB)

• The New MessageBOOSTER™ cDNA Synthesis Kit for qPCR Preserves Transcript Abundance
• Produce High Yields of a High Affinity 2´-F-RNA Aptamer Using the DuraScribe® T7 Transcription Kit
• Control the Length of the 3´-Poly(A)-tail Added to Your RNA Using the A-Plus™ Poly(A) Polymerase Tailing Kit
• Ask Frank
• The High Yield ArrayPure™ Nano-scale RNA Purification Kit Outperforms Kits from Two Other Major Suppliers
• A Rapid Method for Extraction of Rotavirus RNA from Fecal Samples and Genotyping of Rotavirus by Reverse Transcription-PCR
• Get the Highest Yield of 5´-Capped RNA from an In Vitro Transcription Reaction
• Recent Publications Citing the TargetAmp™ aRNA Amplification Kits for Gene Expression Studies
• Tobacco Acid Pyrophosphatase Efficiently Removes the 5´-Cap Structure From Eukaryotic mRNAs
• Impressive Reproducibility of ArrayPure™ RNA Purifications

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Volume 13 * Number 1 * 2006 (3.00 MB)

• TargetAmp™ aRNA Amplification Kits: High Fidelity RNA Amplification for High Quality Microarray Results
• Rapid, High Yield Purification of Plasmids Free of RNA and Genomic DNA
• MessageBOOSTER™ cDNA Synthesis Kit for Real-Time PCR Quantification of Low-Abundance Transcripts from as Little as One Cell
• An Improved CopyControl™ Fosmid Vector Maximizes End-Sequencing Results
• The Generation of Alu-Based DNA Profiles Using Epicentre's FailSafe™ PCR System
• Molecular Analysis of Lactic Acid Bacteria in an Inhospitable Environment: Beer
• Ask Frank
• Enrich Prokaryotic or Eukaryotic mRNA Using Epicentre's Terminator™ 5´-Phosphate-Dependent Exonuclease
• Make Circular ssDNA without "Bridges" or "Splints"

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Volume 12 * Number 1 * 2005 (1.95 MB)

• TargetAmp™ 2-Round Kits: An Improved RNA Linear Amplification System for Microarray Analysis from as Little as 1 Cell
• Eberwine-type Kits Generate High Yields of aRNA from 25 to 500 ng of Total RNA
• Biotin-X-X-NHS, for Biotin Labeling of Aminoallyl-RNA
• Purify RNA from a Single Cell Using the ArrayPure™ Nano-scale RNA Purification Kit
• Ask Frank
• FailSafe™ PROBES Real-Time PCR System Enables Easy, Reliable Optimization of Allelic Descrimination Assays
• TargetAmp™ Aminoallyl-aRNA is a Suitable Template for cDNA Synthesis
• Full-Length Reverse Transcription of >15-kb mRNA Using MonsterScript™ Reverse Transcriptase
• Fecal DNA is PCR-Ready in 50 Minutes Using the ExtractMaster™ Fecal DNA Extraction Kit

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Volume 11 * Number 6 * 2004 (1.96 MB)

• Purify Microbial DNA from Water Samples Using the WaterMaster™ DNA Purification Kit
• Purify RNA from 10 to 10,000 Eukaryotic Cells Using the ArrayPure™ Nano-scale RNA Purification Kit
• DNA Isolation from a Filamentous Fungus Using the MasterPure™ Yeast DNA Purification Kit
• Environmental Genomics Studies Use Epicentre's Fosmid Kits to Generate Libraries
• Quickly Extract DNA from Tissue in 8 Minutes
• Ask Frank
• Transposon-Based Method to Create and Express N-Terminal and C-Terminal Protein Deletions
• Rapidly Screen Bacterial RNA Polymerase Inhibitors Using Innovative Kool™ NC-45™ Technology
• Transcribe ssDNA Templates with Single-Stranded Promoters Using the MiniV™ In Vitro Transcription Kit
• Produce Templates for Rolling Circle Replication and Transcription in 1 Hour Using CircLigase™ ssDNA Ligase

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Volume 11 * Number 5 * 2004 (1.21 MB)

• APex™ Heat-Labile Alkaline Phosphatase Dephosphorylates Any DNA Ends in Just 15 Minutes
• Clone Unstable DNA by Lowering the Copy Number of Common Vectors Using CopyCutter ™ EPI400™ E. coli Cells
• Undergraduates Study Gene Expression in S. cerevisiae Using a Student-Optimized Protocol
• Introducing TAQurate™ Real Time PCR Master Mix, A Single High-Performance Mix
• Ask Frank
• Restriction-Enzyme Screen Clones from Colony to Gel in 25 Minutes or Less
• Colony Fast-Screen™ Kit (Restriction Screen) Identifies Subclones with 124-bp Fragment
• The Oyster Bacterial Biome: Who Else Is Inside the Shell?
• Transposon Construction Vectors
• Tobacco Acid Pyrophosphatase (TAP) Functional Assay Ensures Decapped mRNA

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Volume 11 * Number 4 * 2004 (3.43 MB)

• 3-Minute Protocol for the BuccalAmp™ DNA Extraction Kit
• Customer Focus
• “Fishing” for Genes Encoding Noncytoplasmic Proteins in a Vibrio Fosmid Library
• Ask Frank
• Cloning a Real-Time PCR Product; Does SYBR® Green I Dye Interfere?
• High Throughput Screening For RNAi Using the MessageMuter™ shRNAi Production Kit
• Screen BAC Clones Quickly and Efficiently, Without Not I

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Volume 11 * Number 3 * 2004 (3.55 MB)

• Isolate BAC DNA Pure Enough to Store for One Year Using the BACMAX™ DNA Purification Kit
• Introducing MasterPure™ Gram Positive DNA Purification Kit
• A Rapid Method to Assess Protein Overproduction and Solubility in E. coli Using EasyLyse™ Bacterial Protein Extraction Solution
• Transcribe High Yields of Active Ribozyme in 30 Minutes Using the AmpliScribe™ T7 -Flash™ Transcription Kit
• Ask Frank
• Customer Focus
• Cloning and Expression of Moraxella catarrhalis Adhesins Using the CopyControl™ PCR Cloning System
• Transposon-Based Strategies for Efficient DNA Sequencing and Functional Genomics

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Volume 11 * Number 2 * 2004 (1.89 MB)

• A Novel, Rapid Method to Release Intact Yeast RNA Using the MasterPure™ Yeast RNA Purification Kit
• High Efficiency Real-Time PCR Amplifies a 1.6-kb Template Using the FailSafe™ Real-Time PCR System
• Ask Frank
• Customer Focus
• shRNA Offers Important Advantages Over siRNA for RNAi Studies: MessageMuter™ shRNAi Production Kit
• Generate High Yields of Biotin-Labeled RNA Using the AmpliScribe™ T7-Flash™ or the AmpliScribe™ T3-Flash™ Transcription Kits

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Volume 11 * Number 1 * 2004 (2.99 MB)

• RNAi Studies Made Faster, Easier, and at Lower Cost Using the New MessageMuter™ shRNAi Production Kit
• Improve Your RT-PCR Results Using Thermostable MasterAmp™ Tth DNA Polymerase
• Maximize the Yield of Short RNA Transcripts Using an AmpliScribe™ T7-Flash™ or T3-Flash™ Transcription Kit
• Ask Frank
• Customer Focus
• Prepare DNA from Colonies in 10 Minutes for Real-Time PCR Using the Colony Fast-Screen™ Kit and FailSafe™ Real-Time PCR System
• Get High Stringency and High Specificity Ligation-Dependent Mutation Screening with Ampligase® Thermostable DNA Ligase
• Transcribe 2´-Modified RNAs, Including RNAs Resistant to RNase A, Using Mutant T7 and SP6 R&DNA™ Polymerases
• Determining the Copy Number of Induced CopyControl™ Clones Using the FailSafe™ Real-Time PCR System

Volume 10 * Number 3 * 2003 (4.88 MB)

• The FailSafe™ Real-Time PCR Capillary System: Now Available for Use with Capillary-Based Real-Time PCR Instruments
• DNA Purification from Hard-to-Lyse Bacteria Using Ready-Lyse™ Lysozyme and the MasterPure™ DNA Purification Kit
• Introducing EasyLyse™ Bacterial Protein Extraction Solution to Gently Lyse Cells While Preserving up to 10 Times More Enzyme Activity of Purified Proteins
• Quantifying RNAi-Meditated Gene Silencing by Real-Time RT-PCR Using the MasterAmp™ Real-Time RT-PCR Kit
• Transcribe Milligram Amounts of RNA in as Little as 30 Minutes Using the AmpliScribe™ T7-Flash™ Transcription Kit
• Ask Frank
• Customer Focus
• The EZ::TN™ Transposome™ Complex Simplifies Transposon Mutagenesis in a Wide Variety of Microorganisms
• Brian Wickes, University of Texas Health Science Center at San Antonio, a MasterPure™ Yeast DNA Purification Customer, Outlines His Fungal Research
• Epicentre Completes Validation of New cGMP Compliant Manufacturing Facility

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Volume 10 * Number 2 * 2003 (1.48 MB)

• Rapid, High Yield Transcription of Short Hairpin RNA (shRNA) for RNAi-Mediated Gene Silencing
• High Quality RNA for Use with Microarrays
• MasterPure™ Genomic DNA for Bacillus anthracis Analysis
• DuraScript™ dsRNA Digested Using Human Dicer Enzyme Is As Effective and Specific in RNAi-Mediated Gene Silencing As Canonical "Diced" dsRNA
• Transcribe More RNA in 30 Minutes Than Other In Vitro Transcription Kits Produce in 2 Hours
• Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair Follicles, Tail Snips, Bacterial Cells, or Feathers Using the QuickExtract™ DNA Extraction Solution
• 10 Reasons Why DNA Cloning Kits from Epicentre Enable the Fastest and Most Reliable Results
• Efficient Cloning of Entire Mitochondrial Genomes in Escherichia coli by In Vitro Insertion of a Transposon
• CopyControl™ Cloning Systems Allow Single-Copy Cloning, Then Controlled Induction for High-Copy DNA Production
• The FailSafe™ Real-Time PCR System with SYBR® Green I Dye Provides Shorter Cycle Times and More Consistent PCR Quantitation with Every Template Every Time

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Volume 10 * Number 1 * 2003 (1.21 MB)

• The FailSafe™ Real-Time PCR System Provides Highly Sensitive and Specific Real-Time PCR Analysis
• The MasterAmp™ Real-Time RT-PCR Kit Provides Superior Sensitivity and Consistent Quantitation
• 6 Reasons Why DuraScript™ RNA Is Better for RNAi
• Production of High-Activity Digoxigenin-Labeled Riboprobes for In Situ Hybridization Using the AmpliScribe™ T7 High Yield Transcription Kit
• Transcribe More RNA in 30 Minutes Than the Leading Competitor's "High Yield" Transcription Kit Can Do in 2 Hours
• Obtain the Highest Yield of Capped RNA from an In Vitro Transcription Reaction
• The SoilMaster™ DNA Extraction Kit Provides PCR-Ready Soil DNA in Less Than an Hour
• Which Kit is Best for Your Genomic DNA Cloning Project–the CopyControl™ Fosmid Library Production Kit or the CopyControl™ BAC Cloning Kit?
• Now, There Are Two Ways to Rapidly Screen Your Clones without Cultures, DNA Purifications, or Restriction Digests
• Direct Selection of Genes Encoding Non-Cytoplasmic Proteins
• Efficient Mapping of Functional Domains or Epitopes of Proteins
• A Mu-Based Transposition System that Is At Least 50-Fold More Efficient than the Competition
• Phage T1-Resistant Electrocompetent E.coli with a Transformation Efficiency of >1 X 10¹⁰

Volume 9 * Number 3 * 2002 (912 KB)

• Construction of Four CopyControl™ BAC Libraries by BACTROP - a BAC-Based Platform to Study Tropical Plant Species
• How the CopyControl™ BAC Cloning Kits Work
• Unlike Other PCR Products, the FailSafe™ PCR System Amplifies a GC-Rich Template and a 20-Kb Template on the First Try
• Jumping Green Genes: A Transposon-Based Approach for Rapidly Creating Functional, Flourescent Fusion Proteins
• Simple, Efficient Production of Short Double-Stranded RNA Using RNase III
• RNase III (E.coli) Now Available from Epicentre
• Get Consistently High Yields of Full-Length RNA from Both Long and Short DNA Templates Using AmpliScribe™ T7 High Yield Transcription Kits
• TransforMax™ EC100™ Chemically Competent E.coli Beat the Competition in Transformation Efficiency
• Quick and Efficient Purification of Single- or Double-Stranded RNA Transcripts Using the MasterPure™ RNA Purification Kit
• Obtain the Highest DNA Yields from Yeast with the MasterPure™ Yeast DNA Purification Kit
• Ampligase® Thermostable DNA Ligase
• Get Cleaner Plasmid Preparations with Plasmid-Safe™ ATP-Dependent DNase
• Increase Protein Yield for Proteomics, High-Throughput Screening or Preparative Purification
• Identification of a Potential Virulence-Related Operon by Rescue Cloning EZ::TN™ Transposon Insertion Sites from a Bacterial Pathogen

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Volume 9 * Number 2 * 2002 (1.01 MB)

• Extract PCR-Ready Soil DNA in Less Than an Hour with the New SoilMaster™ DNA Extraction Kit
• Here is How to Never Fail at PCR
• Rapid and Efficient Cloning of Large PCR Products, Up to 15 Kb, Using the New CopyControl™ PCR Cloning Kits
• Rapid Identification of EZ::TN™ Transposon Insertion Sites in the Genome of Neisseria gonorrhoeae
• TypeOne™ Inhibitor Improves Transformation Efficiencies by Blocking Type I Restriction and Modification Systems In Vivo
• An Improved Approach for Construction and Analysis of BAC Libraries Using the CopyControl™ pCC1BAC™ Vector Containing an Inducible High-Copy Origin
• Integrate CopyControl™ Capability into Existing BAC and Fosmid Clones
• Size Your BAC Clones in 4 Hours without Growing Cultures or Performing Restriction Digests
• Isolation of Bovine and Mouse DNA from Buccal and Vaginal Samples Using the BuccalAmp™ DNA Extraction Kit
• Science Students Amplify Human Globin Gene DNA from Buccal Samples
• Get Consistently High Yields of Full-Length RNA From Both Long and Short DNA Templates Using AmpliScribe™ T7 High Yield Transcription Kits

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Volume 9 * Number 1 * 2002 (896 KB)

• Epicentre Revolutionizes Cloning By Introducing CopyControl™ Cloning Systems for Single-Copy Cloning and "On-Demand" Induction of Clones to High Copy Number
• The CopyControl™ Fosmid Library Production Kit Generates More Complete and Unbiased (Blunt-End) Libraries of Stable, 40-Kb Genomic Clones
• Save At Least 2 Days Constructing a CopyControl™ BAC Library Then Amplify the Clones to 10 - 20 Copies Per Cell, for Higher DNA Yields and Purity
• The CopyControl™ PCR Cloning Kits Enable Faster, More Reliable Cloning of Any PCR Product Up to 15 Kb - Even If It Has High A-T, G-C, or Repetitive Sequences, or Encodes a Toxic Peptide
• Retrofit Existing BAC and Fosmid Clones with CopyControl™ Capability Using  a Kit for Simple In Vitro Insertion of an OriV-containing EZ::TN™ Transposon
• BAC-Tracker™ Supercoiled DNA Ladder
• TransforMax™ EPI300™ E. coli
• Electroporation Cuvettes
• Obtain PCR-Ready Genomic DNA from Buccal Cells, HeLa Cells, Hair Follicles, Tail Snips, Bacterial Cells, or Feather Using the QuickExtract™ DNA Extraction Solution
• Transposon Mutagenesis in the Parasitic Protozoa Trypanosoma brucei with the EZ::TN™ Transposome™
• In Vitro Insertion of a Transposon Containing an E. coli Origin of Replication Facilitates Rapid Recovery, Propagation, and Sequencing of Circular DNA Molecules from Heterologous Organisms
• DNA Ligation in 5 Minutes!

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Volume 8 * Number 3 * 2001 (1.59 MB)

• The BuccalAmp™ DNA Extraction Kit: A New Tool for Easier, Faster Sample Collection and Extraction of PCR-Ready Genomic DNA from Large Numbers of Samples
• Get Consistent PCR of Templates 20 Kb to >40 Kb Using the MasterAmp™ Extra-Long PCR System
• Prepare Custom EZ::TN™ Transposons by PCR Using Primers with Transposase-Specific Mosaic End (ME) Sequences
• Cloning-Ready Bacterial Artificial Chromosome (BAC) Vectors
• Get >4X10⁶ BAC Clones/µg DNA Using TransforMax™ EC100™ Electrocompetent E. coli
• Screen Your BAC Clones in 3 Hours and Plasmid Clones in 1 Hour Without Minipreps and Restriction Digests Using the Colony Fast-Screen™ Kit
• Stop Cloning or Subcloning Artifacts!
• Simplify Dephosphorylation of Your Cloning Vectors Using Heat-Labile HK™ Phosphatase
• Sequencing Through GC-rich and AT-rich Sequences with the SequiTherm™ EXCEL™ II DNA Sequencing Kit
• Produce Unbiased Libraries of Stable, Cosmid-Sized Clones Using the EpiFos™ Fosmid Library Production Kit
• Efficiently Clone a PCR Product into the Vector of Your Choice Using the End-It™ DNA End-Repair Kit
• Direct PCR from a Single Bacterial Colony Without DNA Extraction Using the FailSafe™ PCR System
• Get the Highest Yields of Yeast DNA Using the MasterPure™ Yeast DNA Purification Kit
• Create Random Gene Knockouts in Living Cells Using the EZ::TN™ Transposome™
• Rapidly Rescue Clone Transposed Genomic DNA
• DNA Ligation in 5 Minutes! At room temperature.

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Volume 8 * Number 2 * 2001 (1.65 MB)

• EZ::TN™ Transposomes™: A Novel System for Identifying Genes Involved in Bacterial Pathogenesis
• Direct PCR from Dried Blood without DNA Extraction Using the FailSafe™ PCR System
• High Fidelity PCR Amplification of DNA from 20 Kb to >40 Kb Using the MasterAmp™ Extra-Long PCR Kit
• Cloning and Identification of Expressed Plant Defense Genes Following RT-PCR Using the MasterAmp™ High-Fidelity RT-PCR Kit and Degenerate Primers
• New BuccalAmp™ DNA Extraction Kit Revolutionizes Buccal Sample Collection and Processing
• Completely Remove Oligonucleotides and Single-Stranded DNA From Your Reaction Mixes Using Exonuclease I
• Produce Single-Stranded PCR Product for SSCP or Sequencing Using Lambda Exonuclease
• Consistent Production of the Highest RNA Yields with AmpliScribe™ High Yield Transcription Kits
• Highest Yield of 5’-Capped RNA from an In Vitro Transcription Reaction with AmpliCap™ High Yield Message Maker Kits
• Gentle and Quantitative Recovery of Large Genomic DNA Fragments from Agarose Gels for Genomic Cloning
• The Highest Transformation Efficiency Electrocompetent E. coli Cells
• pWEB™ and pWEB::TNC™ Cosmid Cloning Kits and EpiFOS™ Fosmid Library Production Kit
• Use of the EZ::TN™ Transposon Insertion System for High-Throughput Full-Length cDNA Sequencing at the NIH Intramural Sequencing Center (NISC)
• The Most Versatile Transposition System
• DNA Ligation in 5 Minutes! At room temperature.

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Volume 8 * Number 1 * 2001 (3.23 MB)

• Identification of Permissive and Non-Permissive Peptide Insertion Sites in beta-galactosidase using the EZ::TN™ In-Frame Linker Insertion Kit
• Finish your DNA Sequencing Project: Complete Difficult Regions with SequiTherm EXCEL™ II DNA Sequencing Kits
• Rapid Extraction of PCR-Ready DNA from Buccal Cells Using the BuccalAmp™ DNA Extraction Kit
• High Fidelity PCR Amplification of DNA up to 40 Kb Using the MasterAmp™ Extra-Long PCR Kit
• Purification of DNA from Food Materials for Detection of Genetically Modified Organisms (GMOs) Using the MasterPure™ DNA Purification Kit
• RiboShredder™ RNase Blend Destroys Unwanted RNA Quickly and Efficiently
• pIndigoBAC-5 Cloning-Ready Vectors are Extensively Tested for High Cloning Efficiency and Low Background
• Three Ways to Increase Protein Yield for High Throughput Screening, Preparative Purification, or Bioprocessing
• Simultaneous SNP Identification in p53 and HPV Viral Typing with Multiplex Base Excision Sequence Scanning (BESS)
• Efficiently Clone a PCR Product into the Vector of Your Choice Using the End-It™ DNA End-Repair Kit
• DNA Ligation in 5 Minutes, Then Screen Your Clones in 1 Hour Using the Colony Fast-Screen™ Kit
• Produce Unbiased Libraries of Single Copy Cosmid-Sized Clones Using the EpiFos™ Fosmid Library Production Kit
• Whatever the Template, You Will Never Fail at PCR Again. We Promise.

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Volume 7 * Number 4 * 2000 (1.40 MB)

• Direct Sequencing of BAC Clones Without Subcloning or Primer Walking Using EZ::TN™ Transposon Tools
• How to Make Sequencing Faster and Easier Using EZ::TN™ Transposon Tools
• EZ::TN™ Transposon Insertions Into Target DNA In Vitro are Highly Random
• Construction of an Environmental Genomic DNA Library from Soil Using the EpiFOS™ Fosmid Library Production Kit
• New BuccalAmp™ DNA Extraction Kit Revolutionizes Buccal Sample Collection and Processing!
• Cloning Ready BAC Vectors
• EZ::TN™ pMOD™-2<MCS> Transposon Construction Vector Fequently Asked Questions
• Direct PCR from Whole Blood
• Obtain the Highest Transformation Efficiency Possible Using TransforMax™ EC100™ Electrocompetent E. coli
• Rescue Cloning of Bacterial Genomic DNA Using the EZ::TN™ <R6Kgamma-ori /KAN-2> Tnp Transposome™
• EZ::TN™ <R6Kgamma-ori /KAN-2> Insertion Kit
• Electrocompetent E. coli for Rescue Cloning
• DNA Ligation in 5 Minutes at Room Temperature!

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Volume 7 * Number 3 * 2000 (888 KB)

• High-Throughput Screening of Single Nucleotide Polymorphisms (SNPs) Using the BESS-T&G™ Base Reader Kit and the ABI PRISM® 3700 DNA Sequencer
• Protein Modification Using the New EZ::TN™ In-Frame Linker Insertion Kit
• New Transposon Construction Vector for Constructing a Custom EZ::TN™ Transposon
• Recover Intact DNA Up to >2 Mb in Length with GELase™ Agarose Gel-Digesting Preparation
• Exonuclease I:  Highest Activity, Available Now!
• Using the FailSafe™ PCR System to Construct Molecular Tools for the Functional Characterization of a Saccharomyces cerevisiae Gene
• The MasterAmp™ RT-PCR Kit for High Sensitivity Outperforms Other Kits for Sensitive Detection of RNA
• High Efficiency Packaging of Methylated DNA for Genomic Library Construction Using MaxPlax™ Lambda Packaging Extracts
• New High Efficiency TransforMax™ EC100 Electrocompetent E. coli
• DNA Ligation in as little as 5 minutes at room temperature with the Fast-Link™ DNA Ligation Kit

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Volume 7 * Number 2 * 2000 (1.11 MB)

• Using the EZ::TN™ Transposome™ for Transposon Mutagenesis in Mycobacterium smegmatis
• Finish Your DNA Sequencing Project: Complete Difficult Regions with SequiTherm™ EXCEL II DNA Sequencing Kits
• EZ::TN™ Transposome™ Frequently Asked Questions
• Consistent Production of the Highest Possible RNA Yields with Ampliscribe™ High Yield Transcription Kits
• FailSafe™ PCR: A New System For Reliable and Consistent Amplification of Both Routine and Challenging Templates
• EZ::TN ™ Transposon Tools:  EZ::TN™ <T7 /KAN-2> Promoter Insertion Kit and EZ::TN™ In-Frame Linker Insertion Kit
• GELase™ Agarose Gel-Digesting Preparation and Plasmid-Safe™ ATP-Dependent DNase
• MaxPlax™ Lambda Packaging Extracts

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Volume 7 * Number 1 * 2000 (1.41 MB)

• Generating Unidirectional Deletions by In Vitro Transposition for Localizing and Sequencing a Cloned Gene using EZ::TN™ Transposon Tools
• Get the Highest Possible Yield of Full Length RNA with AmpliScribe™
• Highly Efficient Recovery of DNA from Dried Blood Using the MasterPure™ Complete DNA and RNA Purification Kit
• Recover Intact DNA 50 Kb to >2 Mb in Length with GELase™ Agarose Gel-Digesting Preparation
• Two Unique Kits for All Your RT-PCR Needs!
• Detection of Heterozygous Mutations in Hereditary Colorectal Cancer Using the BESS-T™ Base Reader Kit
• DNA Ligation in as Little as 5 Minutes with Fast-Link™ DNA Ligation Kits

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Volume 6 * Number 3 * 1999 (300 KB)

• Use of EZ::TN™ Transposomes™ for Genetic Analysis and Direct Sequencing of Bacterial Genomic DNA
• Get the Highest Possible Yield of Full Length RNA!
• Ultra-Sensitive Purification of Microbial Nucleic Acids Using the MasterPure™ Complete DNA and RNA Purification Kit
• The New MasterAmp™ High Fidelity RT-PCR Kit for Accurate and Flexible RT-PCR
• Superior RT-PCR Fidelity with the MasterAmp™ High Fidelity RT-PCR Kit

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Volume 6 * Number 2 * 1999

• FailSafe™ PCR: A New System For Reliable and Consistent Amplification of Both Routine and Challenging Templates
• New Transposon Tools for Genomics and Proteomics Research
• EZ::TN™ Transposon Tools
• Epidemiological Typing of Viruses Using the BESS-T™ Base Reader Kit: Identification of Genetic Variation Among St. Louis Encephalitis Virus Isolates Using an ABI Automated DNA Sequencer
• Amplification of Human Papillomavirus (HPV) DNA From Gynecological Specimens Isolated Using MasterAmp™ DNA Extraction Solution and PreservCyt® Solution
• FailSafe™ PCR System

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Volume 6 * Number 1 * 1999

• The New MasterPure™ Plant Leaf DNA Purification Kit: Use in Grapevine DNA STR Analysis
• Using MasterAmp™ PCR PreMixes to Optimize Analysis of High GC Content Genes: PCR Amplification of the Melanocortin-1 Receptor Gene
• Faster Genotyping of Transgenic Mice with the MasterPure™ DNA Purification Kit and MasterAmp™ PCR Products
• New Products

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Volume 5 * Number 4 * 1998

• Demystifying DNA Yields from Yeast: Introducing the MasterPure™ Yeast DNA Purification Kit
• Efficient Fractionation of Periplasmic, Cytoplasmic, and Membrane Proteins From Escherichia coli Using the Epicentre PeriPreps™ Periplasting Kit
• Detection of the Differential Expression of Chorionic Gonadotropin by Multiplex RNA Amplification Using the MasterAmp™ RT-PCR Kit
• Detection of a Single-Base Substitution in the Human Calcium Sensing Receptor Gene Using the BESS T-Scan™ Mutation Detection and Localization Kit with an ABI DNA Sequencer
• Q&A: BESS Mutation Detection and Localization Kits

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Volume 5 * Number 3 * 1998

• High-Efficiency Cosmid Library Construction Using the New pWEB™ Cosmid Cloning Kit
• Detection of Blood-Borne Microbes with the MasterPure™ Complete DNA and RNA Purification Kit
• Multiplex PCR Amplification of STRs from DNA Isolated with the MasterAmp™ Buccal Swab DNA Extraction Kit
• Isolation of DNA from Paraffin-Embedded Tissue Using the MasterPure™ Complete DNA and RNA Purification Kit

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Volume 5 * Number 2 * 1998

• Single-Nucleotide DNA Fingerprinting of Hepatitis C Virus Using the BESS T-Scan™ and New BESS G-Tracker™ Mutation Detection and Localization Kits
• Analysis of BESS T-Scan™ Mutation Data Using an ABI Automated DNA Sequencer
• Single-Nucleotide Typing of Mycobacterium Species Using the BESS T-Scan™ Mutation Detection and Localization Kit
• The MasterPure™ Complete DNA and RNA Purification Kit: A Flexible System for Rapid Isolation of DNA and RNA from a Wide Variety of Sources

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Volume 5 * Number 1 * 1998

• One-Step cDNA Synthesis and Amplification with the New MasterAmp™ RT-PCR Kit
• Convenient, Rapid Enrichment of Periplasmic and Spheroplasmic Protein Fractions Using the New PeriPreps™ Periplasting Kit
• Degrading Protein in DNA Samples to Ensure Proper Migration in Agarose Gels

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Volume 4 * Number 4 * 1997

• Detection of Human DNA Mutations Using Base Excision Sequence Scanning (BESS) and the LI-COR® IR² Automated DNA Sequencer
• Use of the MasterAmp™ PCR Optmization Kit in Multiplex PCR to Detect Dystrophin Gene Deletions Characteristic of Duchenne and Becker Muscular Dystrophies
• Rapid Isolation of PCR-Ready DNA for Prenatal Diagnosis Using the MasterAmp™ Buccal Swab DNA Extraction Solution
• In-Gel PCR: DNA Amplification in the Presence of Agarose

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Volume 4 * Number 3 * 1997

• Isolation of Intact, High Molecular Weight DNA Fragments for the E. coli Genome Project
• The ReadyPreps™ Protein Preparation Kit Facilitates Protein Isolation from Bacterial Cells
• The SequiTherm EXCEL™ II DNA Sequencing Kit: A New Kit for Both Cycle and Non-Cycle Sequencing

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Volume 4 * Number 2 * 1997

• Simple, Rapid, Nucleotide-Level Characterization of Sequence Variations Using the BESS T-Scan™ Mutation Detection and Localization Kit
• Increased Sensitivity in DNA Sequencing with the New SequiTherm EXCEL™ II DNA Polymerase
• Sensitive and Efficient PCR Amplification of DNA with AmpliTherm™ DNA Polymerase
• A One-Step MasterAmp™ RT-PCR Procedure to Detect the BCR/ABL Transcript of the Philadelphia Chromosome and the Enterovirus Genome

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Volume 4 * Number 1 * 1997

• New MasterAmp™ PCR Enhancement Technology Consistently Amplifies Even Very Difficult Templates
• Convenient and Highly Reproducible Amplification of DNA from Difficult Templates Using the MasterAmp™ PCR Optimization Kit
• Sensitive and Flexible Amplification of DNA Using the MasterAmp™ PCR Core Kit, Including Amplification of Difficult Templates
• Use of the MasterAmp™ Buccal Swab DNA Extraction Kit for Genomic Marker Amplification
• Rapid Purification of Genomic DNA from Buffy Coat Using the MasterPure™ Genomic DNA Purification Kit
• Optimal Conditions for Digesting Genomic DNA with T4 Endonuclease V
• Resolving Ambiguities in Automated Sequencing Data with the IsoTherm™ DNA Sequencing Kit

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Volume 3 * Number 4 * 1996

• The Direct Sequencing of CCG Repeats from a Cosmid Genomic DNA Template
• An Efficient and Inexpensive Method for Mutant Detection in Mice Transgenic for Bacteriophage Lambda
• Use of RNase I for the Efficient Elimination of RNA from DNA Preparations and Mismatch Detection
• Specificity and Heat Lability of Plasmid-Safe™ ATP-Dependent DNase

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Volume 3 * Number 3 * 1996

• New SequiTherm™ EXCEL™ DNA Sequencing Kits Read Through Even Difficult Template Regions and Decrease Background in DNA Sequencing
• T4 Endonuclease V Detects UV Transilluminator Damage to DNA in Agarose Gels
• High Efficiency Ligation Using the Fast-Link™ DNA Ligation and Screening Kit
• The Advantages of Epicentre's MMLV-RT for Making Full-Length, Double-Stranded cDNA Templates
• Experimental Relevance of the Observed Two-Fold Higher Activity of Epicentre's Thermostable DNA Polymerases
• A Simple Method for RNA Purification from In Vitro Transcription Reactions

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Volume 3 * Number 2 * 1996

• Non-Radioactive In Situ Hybridization Using Digoxigenin-labeled RNA Probes Generated with the AmpliScribe™ T7, SP6, and T3 High Yield Transcription Kits
• Rapid Cloning and Identification of Recombinant Plasmids Using the [Fast-Link™] DNA Ligation and Screening Kit
• A Single Buffer for Multiple Manipulations of DNA Samples
• Tech Tips: DNA Sequencing

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Volume 3 * Number 1 * 1996

• Molecular Chaperones - An Overview
• Tech Tips: Nucleic Acid Ligation
• Protocol Update: Ampligase® Thermostable DNA Ligase for Gene Synthesis
• Selecting the Best Product for Optimum Results in In Vitro Transcription
• Irreversible Heat Inactivation of HK™-UNG Thermolabile Uracil N-Glycosylase

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Volume 2 * Number 5 * 1995

• High Efficiency Transgenic DNA Rescue Using the MutaPlax™ Transgenic Lambda DNA Packaging System
• Production of Biotin-Labeled RNA Probes Using the AmpliScribe™ T7 High Yield Transcription Kit
• DNA Sequencing in a Microtiter Plate Using the IsoTherm™ DNA Sequencing Kit for End-Labeling
• Sequencing a Double-Stranded Clone of a Mutant Human Complement Factor H Gene Using the IsoTherm™ DNA Sequencing Kit for Internal Labeling

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Volume 2 * Number 4 * 1995

• Sequencing a 2.5 Kb CT-Rich Region of the Human Autosomal Dominant Polycystic Kidney (PKD1) Disease Gene Using the SequiTherm™ Long-Read™ Cycle Sequencing Kit
• Mutagenesis of Amplified DNA Sequencing Using Ampligase® Thermostable DNA Ligase
• Tech Tips: Rapid Isolation and Cloning of DNA Inserts
• Tech Tips: Isolation of Size-Specific RNA from Total Yeast RNA Using GELase™ Agarose Gel-Digesting Preparation
• IsoTherm™ DNA Sequencing Kits: New Kit Configuration!

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Volume 2 * Number 3 * 1995

• Repeat Expansion Detection Using Ampligase® Thermostable DNA Ligase
• Obtaining Maximum Packaging Efficiencies in Transgenic DNA Rescue
• Preparation of Competent Cells Using Transformation and Storage Solution
• Tech Tips: Direct Sequencing of PCR Products from an Agarose Gel using the SequiTherm™ Cycle Sequencing Kit

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Volume 2 * Number 2 * 1995

• Rapid Gene Synthesis Using Ampligase® Thermostable DNA Ligase
• Increased Efficiency of Rescue of a Lambda Shuttle Vector from Transgenic Mouse DNA using MaxPlax™ Packaging Extract
• Tech Tips: A Rapid and Efficient Method for Screening Bacterial Colonies for Recombinant Plasmids

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Volume 2 * Number 1 * 1995

• MaxPlax™ Packaging Extract: The Highest Efficiency System for DNA Packaging
• DNase-Free Thermolabile DNA Phosphatase
• Direct Sequencing of P1 Bacteriophage Clones Using SequiTherm™ DNA Polymerase
• Isolation of High Molecular Weight DNA from Soybeans Using GELase™ Gel-Digesting Preparation
• Tech Tips: DNA-Specific Nucleases
• Sequencing "Difficult" DNA Templates

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Volume 1 * Number 4 * 1994

• Use of GELase™ Gel-Digesting Preparation for Improved EM of High Molecular Weight DNA Extracted from an Agarose Gel
• Intermolecular Ligation Using T4 RNA Ligase
• Improved Yields of DNA, RNA, and Proteins with Ready-Lyse™ Lysozyme Solution
• Dephosporylation of RNA Using HK™ Thermolabile Phosphatase
• Tech Tips: AmpliScribe™ T7, SP6, and T3 High Yield Transcription Kits

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Volume 1 * Number 3 * 1994

• Use of GELase™ Gel-Digesting Preparation in Constructing Chromosome-Specific YAC Libraries
• IsoTherm™ DNA Polymerase and IsoTherm DNA Sequencing Kits
• T4 RNA Ligase
• Thermal Cycle Sequencing Directly from Bacterial Colonies Using SequiTherm™ DNA Polymerase
• Sequencing GC-Rich Templates Using SequiTherm™ DNA Polymerase
• Tech Tips: SequiTherm™ Cycle Sequencing Kit for Manual Sequencing of DNA

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Volume 1 * Number 2 * 1994

• Unmodified T7 DNA Polymerase for the In Situ Detection of DNA Fragmentation Associated with Apoptosis
• Cycle Sequencing Using SequiTherm™ with [alpha-³³P]-Nucleotides
• Tobacco Acid Pyrophosphatase to Remove Caps from mRNA
• Use of Plasmid-Safe™ DNase to Prevent Cloning Artifacts Due to Bacterial Chromosomal DNA
• A Simple Method of RNA Purification from In Vitro Transcription Reactions
• Tech Tips: GELase™ Agarose Gel-Digesting Preparation
• GELase™: How to Do the Fast Protocol Even Faster

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Volume 1 * Number 1 * 1994

• SequiTherm™ Long-Read™ Kit for High Volume DNA Sequencing: Use of a Modified M13 DNA Preparation with SequiTherm DNA Polymerase for Fluorescence Sequencing Reactions
• Automated DNA Sequencing Using the SequiTherm™ Long-Read™ Kit
• AmpliScribe™ for Synthesis of Active Ribozymes in Milligram Quantities
• Tech Tips: Plasmid-Safe™ ATP-Dependent DNase
• Cycle Sequencing of Unpurified PCR-Generated DNA Using a New Thermostable DNA Polymerase

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