In Vitro Transcription Kit Selection Guide

Kit Name End Product and Applications RNA Yield Per 1 µg of Control DNA template Reaction time Comments
AmpliScribe™ T7 High Yield Transcription Kit High yields of RNA for many applications 100 µg - 130 µg 2 hours Epicentre's original AmpliScribe Kit
AmpliScribe™ T7-Flash™ Transcription Kit High yields of RNA for many applications 150 µg - 180 µg 30 minutes Produces more RNA in shorter reaction time than other commercial kits.
AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit Biotin-labeled RNA for use as non-radioactive RNA probe 150 µg - 180 µg 2 hours  
DuraScribe® T7 Transcription Kit DuraScript® RNA that is resistant to RNaseA and "finger nucleases". Ideal for RNA Aptamer, ribozyme, RNAi, anti-sense RNA studies. 30 µg - 50 µg 2 - 6 hours Kit includes DuraScribe® T7 RNA Polymerase that incorporates 2´-F-dCTP and 2´-F-dUTP, as well as ATP and GTP into full length DuraScript RNA transcripts that are resistant to RNase A.
Poly(A) Polymerase Tailing Kit Adds a 3´ poly(A)-tail to in vitro transcribed RNA.   30 - 60 minutes Includes Poly(A) Polymerase enzyme, Reaction Buffer and ATP.
RiboGuard™ RNase Inhibitor       Inhibits a variety of RNases including RNaseA.